Cell
Volume 83, Issue 4, 17 November 1995, Pages 589-598
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Article
Hip, a novel cochaperone involved in the eukaryotic hsc70/hsp40 reaction cycle

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Abstract

The Hsc70-interacting protein Hip, a tetratricopeptide repeat protein, participates in the regulation of the eukaryotic 70 kDa heat shock cognate Hsc70. One Hip oligomer binds the ATPase domains of at least two Hsc70 molecules dependent on activation of the Hsc70 ATPase by Hsp40. While hydrolysis remains the rate-limiting step in the ATPase cycle, Hip stabilizes the ADP state of Hsc70 that has a high affinity for substrate protein. Through its own chaperone activity, Hip may contribute to the interaction of Hsc70 with various target proteins. We propose a mechanism for the regulation of eukaryotic Hsc70 that is distinct from that of bacterial Hsp70. The Hsc70/Hsp40/Hip system is apparently independent of a GrpE-like nucleotide exchange factor.

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Present address: Zentrum für Molekulare Biologie, Universität Heidelberg, D-69120 Heidelberg, Federal Republic of Germany.