Identification of penicillin allergenic determinants that bind IgE antibodies in the sera of subjects with penicillin allergy

Abstract

Much of the literature on penicillin hypersensitivity is devoted to the identification of penicillin antigens rather than allergens. Human IgE-binding determinants on different penicillins have rarely been closely investigated with the view of defining fine structural allergenic features and differences. We have developed radioimmunoassays employing ampicillin, amoxicillin and ticarcillin solid phases for the detection of penicillin-reactive IgE antibodies. Quantitative hapten inhibition studies employed to identify IgE-binding regions on the penicillin molecules revealed a heterogeneous group of allergenic determinants consisting exclusively, or in part, of the α-aminobenzyl and benzyl side chain groups and the β-lactam and thiazolidine rings of the penicillin nucleus.