Elsevier

Peptides

Volume 15, Issue 4, 1994, Pages 675-681
Peptides

Article
Glycosylation of the GLP-1 receptor is a prerequisite for regular receptor function

https://doi.org/10.1016/0196-9781(94)90095-7Get rights and content

Abstract

The GLP-1 receptor on RINm5F cells is a glycoprotein with a Mr of 63,000. Treatment of the receptor with glycopeptidase F generated a protein with a Mr of 51,000, indicating that the GLP-1 receptor contains N-linked glycans. Tunicamycin pretreatment concentration-dependently decreased GLP-1 binding to RINm5F cells due to a decreased receptor number without change of receptor affinity. Tunicamycin exerted no effect on the GLP-1 receptor mRNA expression. The stimulation of cAMP production was decreased in tunicamycin-treated cells. Our data show that glycosylation of the GLP-1 receptor is a precondition for regular receptor function.

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