Elsevier

Neuroscience Letters

Volume 89, Issue 2, 29 June 1988, Pages 133-139
Neuroscience Letters

Binding sites for 125I-cholecystokinin in primate spinal cord are of the CCK-A subclass

https://doi.org/10.1016/0304-3940(88)90369-2Get rights and content

Abstract

Cholecystokinin (CCK) receptor binding was measured in sections of human, monkey and rat spinal cord using autoradiographical techniques. In each species, high levels of specific 125I-Bolton Hunter CCK binding were detected in the superficial layers of the dorsal horn (the substantia gelatinosa). In monkey and human but not rat spinal cord, 125I-CCK binding was dose-dependently inhibited by low concentrations of the selective CCK-A antagonist L-364,718. Binding of [3H]L-364,718, which was saturable (Bmax = 29.0 ± 0.95 pmol/g wet wt.) and of high affinity (pKd = 9.92 ± 0.16) was also detected in sections of monkey spinal cord and had a similar localization to that of specific 125I-CCK binding. These data indicate that in striking contrast to CCK receptors in rat spinal cord, those in the primate cord are of the CCK-A receptor subclass.

References (26)

  • L.A. Chiodo et al.

    Proglumide: selective antagonism of excitatory effects of cholecystokinin in central nervous system

    Science

    (1983)
  • J.N. Crawley

    Comparative distribution of cholecystokinin and other neuropeptides: why is this neuropeptide different from all other neuropeptides?

    Ann. N.Y. Acad. Sci.

    (1985)
  • M. Denavit-Saubie et al.

    The effects of cholecystokinin-8 in the nucleus tractus solitarius

    Ann. N.Y. Acad. Sci.

    (1985)
  • Cited by (0)

    View full text