Determination of the cannabinoid receptors in mouse × rat hybridoma NG108-15 cells and rat GH4C 1 cells☆
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Cited by (16)
CB<inf>1</inf> and CB<inf>2</inf> Receptor Pharmacology
2017, Advances in PharmacologyCitation Excerpt :The rat and human receptors are highly conserved, 93% identity at the nucleic acid level and 97% at the amino acid level. Similarly, the mouse and rat clones have 95% nucleic acid identity (100% amino acid identity) and the mouse and human clones have 90% nucleic acid identity (97% amino acid identity) (Abood, Ditto, Noel, Showalter, & Tao, 1997; Chakrabarti, Onaivi, & Chaudhuri, 1995; Ho & Zhao, 1996). A meta-analysis of the literature examining cannabinoid ligand-binding affinity revealed subtle interspecies differences for the binding affinities of some ligands (Δ9-THC, CP55,940, WIN55,212-2, SR141716A) for rat vs human CB1 receptors (McPartland, Glass, & Pertwee, 2007).
Spice drugs are more than harmless herbal blends: A review of the pharmacology and toxicology of synthetic cannabinoids
2012, Progress in Neuro-Psychopharmacology and Biological PsychiatryCitation Excerpt :Another patient presented with emesis, agitation, mydriasis, mild tachycardia and seizure activity one hour after smoking SpicyXXX (Simmons et al., 2011b). Importantly, in NG 108‐15 cells, a cell line expressing functional cannabinoid receptors (Ho and Zhao, 1996; Matsuda et al., 1990), the synthetic cannabinoids CP-55,940, CP-47,497 and CP-47,497-C8 have been recently found to be cytotoxic, as they induced apoptosis in a dose-dependent manner likely activating the caspase cascade and involving CB1 receptors, but not CB2 receptors (Tomiyama and Funada, 2011). While the acute adverse effects of synthetic cannabinoids are recognized and documented, there is no information about the chronic use and toxicity of synthetic cannabinoids.
Cannabinoid receptors: Nomenclature and pharmacological principles
2012, Progress in Neuro-Psychopharmacology and Biological PsychiatryCitation Excerpt :The rat and human receptors are highly conserved, 93% identity at the nucleic acid level and 97% at the amino acid level. Similarly, the mouse and rat clones have 95% nucleic acid identity (100% amino acid identity) and the mouse and human clones have 90% nucleic acid identity (97% amino acid identity) (Abood et al., 1997; Chakrabarti et al., 1995; Ho and Zhao, 1996). A molecular phylogenetic analysis which included the CB1 receptor showed that the sequence diversity in 62 mammalian species varied from 0.41 to 27% (Murphy et al., 2001).
Cytotoxicity of synthetic cannabinoids found in "Spice" products: The role of cannabinoid receptors and the caspase cascade in the NG 108-15 cell line
2011, Toxicology LettersCitation Excerpt :In the current study, we examined the cytotoxicity of CP-47,497 and CP-47,497-C8, which are detected in “Spice”, as well as the cytotoxicity of CP-55,940, which is a compound in which substitutions have been made at position C-4 of the cyclohexanol ring of CP-47,497, using a neuroblastoma-glioma hybrid cell line, NG 108-15. The NG 108-15 cell line was used for these studies since it is known to express functional cannabinoid receptors (Ho and Zhao, 1996; Matsuda et al., 1990). Endogenous expression of cannabinoid receptors in these cells allows investigation of whether activation of these receptors can directly regulate synthetic cannabinoid-induced cytotoxicity.
Intracellular cannabinoid type 1 (CB1) receptors are activated by anandamide
2011, Journal of Biological ChemistryCitation Excerpt :In addition, pretreatment with U73122, a phospholipase C (PLC) inhibitor (29), reduced but did not abolish the Ca2+ response to anandamide (Fig. 6C). Next, to validate our results obtained in CB1 receptor-transfected cells in endogenously expressing cells, we carried out calcium imaging experiments in NG108-15 cells, which endogenously express CB1 but not CB2 receptors (17). In NG108-15 cells, extracellular administration of anandamide did not produce a significant increase in [Ca2+]i, whereas the administration of ATP (10 μm) increased [Ca2+]i by 432 ± 57 nm (n = 52) (Fig. 7).
Pharmacology of cannabinoid CB<inf>1</inf> and CB<inf>2</inf> receptors
1997, Pharmacology and Therapeutics
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Accession numbers for the mouse and rat CB 1 receptors are u40709 and u40395.