Neuron
Volume 7, Issue 1, July 1991, Pages 81-89
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Article
A long-term depression of AMPA currents in cultured cerebellar purkinje neurons

https://doi.org/10.1016/0896-6273(91)90076-CGet rights and content

Abstract

Cerebellar long-term depression (LTD) is a model of synaptic plasticity in which conjunctive stimulation of parallel fiber and climbing fiber inputs to a Purkinje neuron induces a persistent depression of the parallel fiber-Purkinje neuron synapse. We report that an analogous phenomenon may be elicited in the cultured mouse Purkinje neuron when iontophoretic glutamate application and depolarization of the Purkinje neurons are substituted for parallel fiber and climbing fiber stimulation, respectively. The induction of LTD in these cerebellar cultures requires activation of both ionotropic (AMPA) and metabotropic quisqualate receptors, together with depolarization in the presence of external Ca2+. This postsynaptic alteration is manifest as a depression of glutamate or AMPA currents, but not aspartate or NMDA currents. These results strengthen the contention that the expression of cerebellar LTD is at least in part postsynaptic and provide evidence that activation of both ionotropic and metabotropic quisqualate receptors are necessary for LTD induction.

References (47)

  • M. Kano et al.

    The glutamate receptor subtype mediating parallel fibre-Purkinje cell transmission in rabbit cerebellar cortex

    Neurosci. Res.

    (1988)
  • G. Levi et al.

    Putative amino acid transmitters in the cerebellum. I. Depolarization induced release

    Brain Res

    (1982)
  • O.J.J. Manzoni et al.

    The glutamate receptor of the Qp type activates protein kinase C and is regulated by protein kinase C

    Neurosci. Lett.

    (1990)
  • K. Murakami et al.

    Protein kinase C activation by cis-fatty acid in the absence of Ca2+ and phospholipids

    J. Biol. Chem.

    (1986)
  • F. Nicoletti et al.

    Pertussis toxin inhibits signal transduction at a specific metabolotropic glutamate receptor in primary cultures of cerebellar granule cells

    Neuropharmacology

    (1988)
  • E. Palmer et al.

    TransACPD, a selective agonist of the phosphoinositide-coupled excitatory amino acid receptor

    Eur. J. Pharmacol.

    (1989)
  • M.E. Sandoval et al.

    Evaluation of glutamate asa neurotransmitter of cerebellar parallel fibers

    Neuroscience

    (1978)
  • D. Schoepp et al.

    Pharmacological and functional characteristics of metabotropic excitatory amino acid receptors

    Trends. Pharmacol. Sci.

    (1990)
  • M.S. Shearman et al.

    Selective activation of the gamma subspecies of protein kinase C from bovine cerebellum by arachudonic acid and its lipoxygenase metabolites

    FEBS Lett.

    (1989)
  • H. Sugiyama et al.

    Glutamate receptor subtypes may be classified into two major categories: a study on Xenopus oocytes injected with rat brain mRNA

    Neuron

    (1989)
  • E. Audinat et al.

    Responses to excitatory amino acids of Purkinje cells and neurones of the deep nuclei in cerebellar slice cultures

    J. Physiol.

    (1990)
  • D.S. Bredt et al.

    Localization of nitric oxide synthase indicating a neural role for nitric oxide

    Nature

    (1990)
  • N.C. Campbell et al.

    Interaction between responses in Purkinje cells evoked by climbing fibre impulses and parallel fibre volleys in the cat

    J. Physiol.

    (1983)
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