The involvement of the small GTP-binding protein Rab5a in neuronal endocytosis

doi:10.1016/0896-6273(94)90456-1

Neuron

Volume 13, Issue 1, July 1994, Pages 11-22

https://doi.org/10.1016/0896-6273(94)90456-1 Get rights and content

Abstract

Rab5a is a small GTPase that regulates fusion of endocytic vesicles to early endosomes. We investigated whether Rab5a is involved in early endocytic traffic in both the axonal and the somatodendritic domains of polarized neurons. Using immunofluorescence, endogenous Rab5a was detected in axons and dendrites. Its localization in axons strongly overlapped that of the synaptic vesicle protein synaptophysin. Indeed, Rab5a coimmunoisolated with synaptophysin-containing vesicles, and antibodies against Rab5a labeled synaptic vesicle-like structures in nerve terminals. The functional association of Rab5a with dendritic and axonal early endosomes was assayed by electron microscopy after overexpression of wild-type and mutant Rab5a in cultured hippocampal neurons. This induced the formation of abnormal endosomes in both the somatodendritic and the axonal domains. These results show a role for Rab5a in axonal and dendritic endocytosis, and the presence of Rab5a on synaptic vesicles indicates that the axonal endosomes participate in the biogenesis of these vesicles.

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Neuron

ArticleThe involvement of the small GTP-binding protein Rab5a in neuronal endocytosis

Abstract

Cell

Cell

Cell

Cell

Cell Biol. Int. Rep.

Curr. Opin. Cell Biol.

Gene

Neuron

Cell

Trends Cell Biol.

Trends Cell Biol.

Curr. Opin. Cell Biol.

Neuron

Cell

Neuron

Cell

Curr. Opin. Cell Biol.

Electron microscopic studies of axonal and dendritic development of hippocampal neurons in culture. II. Synaptic relationships

J. Neurosci.

Synaptic vesicle membrane proteins interact to form a multimeric complex

J. Cell Biol.

Endocytosis in filter-grown Madin-Darby canine kidney cells

J. Cell Biol.

Growth of a rat neuroblastoma cell line in serum-free supplemented medium

Transcytosis in MDCK cells: identification of glycoproteins transported bidirectionally between both plasma membrane domains

J. Cell Biol.

rab5a is a common component of the apical and basolateral endocytic machinery in polarized epithelial cells

Colocalization of synaptophysin with transferrin receptors: implications for receptor biogenesis

J. Cell Biol.

Comprehensive 2 D gel protein databases offer a global approach to the analysis of human cells: the transformed amnion cells (AMA) master database and its link to genome DNA sequence data

Electrophoresis

Molecular cloning of YPT/SEC4-related cDNAs from an epithelial cell line

Mol. Cell. Biol.

The establishment of polarity by hippocampal neurons in culture

J. Neurosci.

Polarized sorting of glypiated proteins in hippocampal neurons

Nature

A small GTP-binding protein (rab3A) dissociates from synaptic vesicles during exocytosis

Nature

Transferrin receptor polarity and recycling accuracy in“tight” and “leaky” strains of Madin-Darby canine kidney cells

J. Cell Biol.

Rat hippocampal neurons in low-density culture

Fine Structure Immunocytochemistry

Article
The involvement of the small GTP-binding protein Rab5a in neuronal endocytosis