Cationic liposome-mediated gene delivery: Biophysical study and mechanism of internalization
Section snippets
Materials
The cationic lipid DC-6-14 was a generous gift from Daiichi Pharmaceutical Co., Ltd. (Tokyo, Japan). DOPE was kindly donated by the Nippon Oil and Fats Co., Ltd. (Tokyo, Japan). Cholesterol was purchased from Wako Pure Chemicals (Osaka, Japan). The DNA expression plasmid pGL3-Control under SV40 promoter and enhancer and luciferase reporter gene were purchased from Promega (Madison, WI). Rh-PE and NBD-PE came from Avanti Polar Lipids (Alabster, AL). Antimycin A, NaF, NaN3, and Z-Phe-Phe-Gly were
Effect of (+/−) charge ratio on lipoplex physico chemical properties
Significant changes occur in the physico chemical features of cationic liposomes upon complexation with the negatively charged DNA [18], [19]. These changes are likely to alter the interaction of cationic liposomes with cell membranes. The zeta potential of the cationic liposomes and lipoplexes provides a measure of their net charge, while dynamic light scattering gives their mean particle size.
Fig. 1 shows the values obtained for mean particle size and zeta potential of lipoplexes at different
Discussion
Despite the growing body of research on the biophysical and biochemical factors involved in cationic liposome-mediated gene transfer, such as structural features [20], [21], cellular association [12], [21], and intracellular trafficking [13], the relationships between these factors and the mechanism(s) of lipoplex internalization into cells are not yet fully understood. Deep understanding of these factors is of great interest for developing efficient lipid-mediated gene delivery. In the present
References (25)
- et al.
Adv. Drug Deliv. Rev.
(1996) - et al.
J. Biol. Chem.
(1995) - et al.
Biochim. Biophys. Acta
(1995) - et al.
J. Biol. Chem.
(1995) - et al.
Biochim. Biophys. Acta
(1995) - et al.
FEBS Lett.
(1994) - et al.
Biophys. J.
(1997) - et al.
J. Biol. Chem.
(1997) - et al.
Biochim. Biophys. Acta
(1999) Science
(1995)
Cancer Surv.
Proc. Natl. Acad. Sci. USA
Cited by (169)
A radiolabeled nanoparticle probe coated with hyaluronic acid via electrostatic interaction to diagnose CD44-positive tumors
2022, Journal of Drug Delivery Science and TechnologyEssential cues of engineered polymeric materials regulating gene transfer pathways
2022, Progress in Materials ScienceA comprehensive review on immuno-nanomedicine for breast cancer therapy: Technical challenges and troubleshooting measures
2022, International ImmunopharmacologyLipid nanoparticles fuse with cell membranes of immune cells at low temperatures leading to the loss of transfection activity
2020, International Journal of PharmaceuticsArginine-modified chitosan complexed with liposome systems for plasmid DNA delivery
2020, Colloids and Surfaces B: BiointerfacesCitation Excerpt :Liposomes also have been widely used for transfections in vitro and in vivo[3,16] and some of them have already been used in clinical trials of gene therapy [17]. One of reasons for efficient transfection is probably due to the unique mechanism of lipoplexes internalization, whose lipoplex interaction with the cell membrane causes fusion that facilitates the uptake of large lipoplex particles through the endosomal pathway [18–20]. Based on the beneficial properties of chitosan and liposome as gene carriers, here we designed a ternary complex formed with a functionalized chitosan associated with a liposome and a pDNA.