A new family of endogenous “big” met-enkephalins from bovine adrenal medulla: purification and structure of docosa- (BAM-22P) and eicosapeptide (BAM-20P) with very potent opiate activity

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Summary

Two novel “big” Met-enkephalins,6a docosa-(BAM-22P) and an eicosapeptide(BAM-20P), both of which yield [Arg6]-Met-enkephalin upon trypsinization, have been purified from the bovine adrenal medulla. The complete sequence of BAM-22P has been established, while BAM-20P has also been proved to be a C-terminal shortened peptide of BAM-22P. Both peptides contain BAM-12P sequence, that previously determined, at their N-terminals with progressive C-terminal extension. The identification of BAM-22P and -20P along with BAM-12P in the tissue presented a new family of “big” Met-enkephalins indicative of the unique feature of enkephalin biosynthesis. In addition, BAM-22P and -20P have extraordinarily potent opiate activity in guinea-pig ileum assay.

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