Elsevier

Neuroscience Research

Volume 44, Issue 2, October 2002, Pages 225-229
Neuroscience Research

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Amygdala kindling induces upregulation of mRNA for NKCC1, a Na+, K+–2Cl cotransporter, in the rat piriform cortex

https://doi.org/10.1016/S0168-0102(02)00093-7Get rights and content

Abstract

GABA, the main inhibitory neurotransmitter in the brain, elicits a hyperpolarizing response by activation of the GABAA-receptor/chloride-channel complex under conditions of normal Cl homeostasis. Thus the pathogenesis of epilepsy could involve an impairment of GABAA-receptor-mediated inhibition due to a collapse of the Cl gradient. We examined the expression patterns of Cl transporters and a Cl channel in a rat amygdala-kindling model. Activity-dependent increases were observed in the mRNA for NKCC1, an inwardly-directed Cl transporter, in the piriform cortex. This suggests that an increase in [Cl]i and a resultant reduction in GABAergic inhibition may occur in the kindled piriform cortex.

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Acknowledgements

We thank Dr R. Timms for language-editing this manuscript. This work was supported by Grants-in-Aid for Scientific Research #12557077 and #12210074 and #13210065 in the Priority Areas (C)-Advanced Brain Science Project from the Ministry of Education, Science, Sports, Culture, and Technology, Japan, and by grants from the Ministry of Health, Welfare and Labor, Japan, and from the Ichiro Kanehara Foundation to A.F.

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