Alterations in the turnover rate of dopamine D1 but not D2 receptors in the adult rat neostriatum after a neonatal dopamine denervation
Section snippets
Neonatal 6-OHDA injections and tissue preparation.
Female Sprague–Dawley rats (Charles River; Montreal, Quebec) were purchased pregnant and individually housed under a fixed light/dark cycle with free access to food and water. All animal use procedures were in strict accordance with the Canadian Council on Animal Care Guide for the Care and Use of Experimental Animals, and all protocols were approved by the Comité de déontologie pour l'expérimentation animale of the Université de Montréal, and the Comité Institutionnel pour la Protection des
Dopamine denervation
As expected, the neonatal 6-OHDA administration resulted in profound reductions in the levels of neostriatal DA and its major metabolites DOPAC and HVA 3 months after lesion, as compared to sham-lesioned rats (Table 1). Interestingly, acute EEDQ treatment in adult rats (3 months old) that had received a sham-lesion also significantly reduced the concentration of striatal DA and DOPAC compared to animals that had received an injection of saline in place of EEDQ. In contrast, no significant
Discussion
The present study demonstrated significant decreases in the rate of repopulation kinetics of dopamine D1 receptors in the rostral neostriatum of adult rats subjected to severe nigrostriatal 6-OHDA denervation as neonates. This decreased rate of receptor synthesis and degradation was associated with an overall decrease in D1 receptor binding. In contrast, no significant differences were found in the repopulation kinetics of neostriatal D2 receptor between 6-OHDA and sham-lesioned animals,
Acknowledgements
This study was funded by a summer studentship from the Natural Sciences and Engineering Research Council of Canada (M. P.), and a Scholarship and Establishment Grant (K. M. D.) from the Fonds de la recherche en santé du Québec, as well as by grant MT-12966 (T. A. R.) from the Medical Research Council of Canada. The authors wish to acknowledge the excellent technical assistance of Louise Grondin and Patricia Bonnot.
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