Elsevier

Biochimie

Volume 56, Issues 11–12, 28 February 1975, Pages 1603-1611
Biochimie

Characterization of differentiated and dedifferentiated clones from a rat hepatoma(†)

https://doi.org/10.1016/S0300-9084(75)80286-0Get rights and content

Summary

An analysis of clonal variability of derivatives of the rat hepatoma line H4IIEC3 has shown that the overwhelming majority of clones express in a stable fashion a number of liver specific functions, including secretion of serum albumin, activity of the liver specific isozymes of alcohol dehydrogenase (EC1.1.1.1) and aldolase (EC4.1.2.13), and high basal activity and hormone inducibility of tyrosine aminotransferase (EC2.6.1.5) and alanine aminotransferase (EC2.6.1.2). The differences in level of expression of these functions cover a range of five to ten-fold, and the variations do not appear coordinated within or between clones.

Seven clones, which differ from the above ones both in morphology and in the expression of liver specific functions, have been isolated. In five of them, no expression of any of the functions is detectable, while two of them show diminished but significant expression of two or three of the functions. In addition, an unexplained negative correlation between activity of glucose-6-phosphate dehydrogenase (EC1.1.1.49) and the expression of liver specific functions is described.

Résumé

L'analyse de la variabilité des clones dérivés de la souche d'hépatome de rat H4IIEC3 a montré que la très grande majorité de ceux-ci expriment de façon stable un certain nombre de fonctions spécifiques de la différenciation hépatique, notamment la sécrétion de sérum albumine, l'activité des iso-enzymes spécifiques du foie de l'alcool déshydrogénase (EC.1.1.1.1) et de l'aldolase (EC.4.1.2.13), le haut niveau de base et l'inductibilité par les hormones stéroïdes de la tyrosine aminotransférase (EC.2.6.1.5) et de l'alanine aminotransférase (EC.2.6.1.2). Les différences entre les clones des niveaux d'expression de ces fonctions sont d'un facteur cinq à dix et ces variations ne paraissent pas être coordonnées.

Sept clones distincts des précédents par leurs morphologies et l'expression des fonctions différenciées ont été isolés. Cinq d'entre eux n'expriment aucune de ces fonctions, et deux autres en expriment deux ou trois à un taux réduit mais significatif. De plus, une corrélation inverse, qui reste inexpliquée, entre l'activité glucose-6-phosphate déshydrogénase (EC.1.1.1.49) et l'expression des fonctions différenciées est décrite.

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    (†)

    This article is dedicated to the memory of Dr. Denise Luzzati.

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