Cancer Letters

Cancer Letters

Volume 112, Issue 1, 15 January 1997, Pages 23-31
Cancer Letters

Lung resistance protein (LRP) expression in human normal tissues in comparison with that of MDR1 and MRP

https://doi.org/10.1016/S0304-3835(96)04542-9Get rights and content

Abstract

MDR1 (P-glycoprotein), multidrug resistance-associated protein (MRP) and lung resistance protein (LRP) are associated with multidrug resistance in various cancer cells. It is known that P-glycoprotein and MRP are also expressed in several normal tissues. However, the exact location of LRP in normal tissues is still unclear. In order to obtain more insight into the physiological role of LRP, its expression in human normal tissues was examined by an immunohistochemical technique, using one monoclonal antibody, LRP-56. Reverse transcriptase-polymerase chain reaction (RT-PCR) was also utilized for several cell lines and fresh-frozen tissues. P-glycoprotein was found to be expressed in the kidney, adrenal, brain vessels, muscle, lung, pancreas, liver, intestine, placenta and testis. MRP was expressed in the kidney, adrenal, lung, pancreas, muscle, intestine, thyroid and prostate, and its distribution mostly overlapped with that of P-glycoprotein. Interestingly, MRP was not expressed in the liver. LRP at 110 kDa was expressed in the kidney, adrenal, heart, lung, muscle, thyroid, prostate, bone marrow and testis. These findings suggest that LRP as well as P-glycoprotein and MRP plays distinct roles in the physiology of various organs.

References (30)

  • HsuS.M. et al.

    Use of avidinbiotin-peroxidase complex (ABC) in immunoperoxidase techniques

    J. Histochem. Cytochem.

    (1981)
  • IzquierdoM.A. et al.

    Prognostic significance of the drug resistance associated protein LRP in advanced ovarian carcinoma

    Ann. Oncol.

    (1994)
  • KrishnamacharyN. et al.

    The MRP gene associated with a non-P-glycoprotein multidrug resistance encodes a 190-kDa membrane bound glycoprotein

    Cancer Res.

    (1993)
  • ManiatisT. et al.

    Molecular Cloning

    (1982)
  • MechetnerE.B. et al.

    Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody

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    Present address: Pathology Research Division, The Research Institute of Tuberculosis, 3-1-24 Matsuyama, Kiyose, Tokyo 204, Japan. Fax: +81 424 924600.

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