Elsevier

Neuroscience Letters

Volume 219, Issue 2, 22 November 1996, Pages 119-122
Neuroscience Letters

Low stringency hybridization study of the dopamine D4 receptor revealed D4-like mRNA distribution of the orphan seven-transmembrane receptor, APJ, in human brain

https://doi.org/10.1016/S0304-3940(96)13198-0Get rights and content

Abstract

We recently reported that the density of mRNA for the dopamine D4 receptor was extremely low in human cerebral cortex but unexpectedly higher in the corpus callosum and spinal cord both of which contain substantial white-matter area. Under low stringency conditions, Northern blot analysis using the D4 probe detected cross-hybridized mRNAs having a similar distributional profile to the D4 mRNA in human brain regions, suggesting the mRNA distributional profile is not peculiar to the D4 receptor. Homology screening revealed one of the mRNAs to be an orphan seven-transmembrane receptor, APJ, abundantly expressed in the corpus callosum and spinal cord. In porcine spinal cord the APJ mRNA was detected at a higher level in white-matter rather than in gray-matter area. These data suggest that a group of seven-transmembrane receptors, including the D4 and AN receptor, is preferentially expressed in the white-matter area probably in non-neuronal glial cells.

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    However, activated or non-activated APJ may affect Ang II-AT1 signaling (Sun et al., 2011). Apelin/APJ system also are distributed in central nerve system, spanal cord (Matsumoto et al., 1996; O'Carroll et al., 2000; Reaux et al., 2001) hypothalamus, cortex, cerebellum, hippocampus, midbrain, striatum, pituitary (Lee et al., 2000; Medhurst et al., 2003), amygdale, raphe nucleus (Reaux et al., 2002) and peripheral areas such as heart, kidney, skeletal muscle, liver, lung, intestine, colon and glands (Habata et al., 1999; Hekmat et al., 2011; Hosoya et al., 2000; Kawamata et al., 2001; Najafipour et al., 2012; O'Carroll et al., 2000; Wang et al., 2004). Interestingly, apelin plays an important role in the neuronal signaling pathway (O'Carroll et al., 2000).

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