Biochemical and Biophysical Research Communications
PCSK9 is expressed in pancreatic δ-cells and does not alter insulin secretion
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Material and methods
Hormone immunoassays. Insulin content and secretion were assessed in mice isolated islets by radioimmunoassay (Linco Research, St. Charles, Missouri, USA). Plasma insulin levels in mice were measured by ELISA (Crystal Chem Inc., Chicago, Illinois, USA). Secreted insulin and insulin content of human islets were assessed with an immunoradiometric assay (Bi-Insulin IRMA, SANOFI Diagnostics Pasteur, Marnes-la-Coquette, France). Somatostatin secretion of isolated islets was measured using an EIA kit
PCSK9 is expressed in human pancreatic δ-cells
First, we verified the expression of PCSK9 mRNA in isolated pancreatic islets from Pcsk9+/+ male mice. Real time Q-PCR analysis showed that the expression of PCSK9 in mouse isolated islets was about 30% of that detected in mouse liver (Fig. 1A). Next, we investigated the expression of PCSK9 in the human pancreas. While PCSK9 mRNA was detected in human islets from pancrease donors (n = 3), PCSK9 was not expressed in the human pancreatic carcinoma cell line Panc1 (Fig. 1B).
To further confirm the
Acknowledgments
This work was supported by Agence Nationale de la Recherche («Physiopathologies humaines 2006 R0651ONS»), Fondation de France, Fondation Cœur et Artères, and ALFEDIAM. C. Langhi is a recipient of a fellowship from the Nouvelle Société Française d’Athérosclérose. C. Le May was supported by a grant from the Fondation pour la Recherche Médicale. P. Costet and B. Cariou are titulars of a Contrat d’interface INSERM-CHU de Nantes. We acknowledge Dr. Philippe Lefebvre (INSERM U545) for providing us
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2021, Biochimica et Biophysica Acta - Molecular and Cell Biology of LipidsCitation Excerpt :Though PCSK9 transcripts are well expressed in insulin-secreting insulinoma cells [4], and human and mouse isolated islets, PCSK9 protein was reported to be expressed in δ-cells [26,27]. It was also shown that mice lacking whole body PCSK9 had higher levels of surface LDLR in islets [26,28]. Several studies using the first reported PCSK9 KO mouse model [15] provided contradictory results regarding the role of PCSK9 in glucose homeostasis [26,27,29].
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These authors contributed equally to this work.