Cell Reports
Volume 8, Issue 5, 11 September 2014, Pages 1271-1279
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Report
Microglia Modulate Wiring of the Embryonic Forebrain

https://doi.org/10.1016/j.celrep.2014.07.042Get rights and content
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Highlights

  • Specific localization of microglia in the embryonic forebrain is observed

  • Early blockade of Csf1R transiently depletes embryonic microglia

  • Microglia modulate dopaminergic axon outgrowth and cortical interneuron positioning

  • Microglial activity in embryonic forebrain involves cx3cr1 signaling

Summary

Dysfunction of microglia, the tissue macrophages of the brain, has been associated with the etiology of several neuropsychiatric disorders. Consistently, microglia have been shown to regulate neurogenesis and synaptic maturation at perinatal and postnatal stages. However, microglia invade the brain during mid-embryogenesis and thus could play an earlier prenatal role. Here, we show that embryonic microglia, which display a transiently uneven distribution, regulate the wiring of forebrain circuits. Using multiple mouse models, including cell-depletion approaches and cx3cr1−/−, CR3−/−, and DAP12−/− mutants, we find that perturbing microglial activity affects the outgrowth of dopaminergic axons in the forebrain and the laminar positioning of subsets of neocortical interneurons. Since defects in both dopamine innervation and cortical networks have been linked to neuropsychiatric diseases, our study provides insights into how microglial dysfunction can impact forebrain connectivity and reveals roles for immune cells during normal assembly of brain circuits.

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

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Co-senior author