Expression patterns of the Tmem16 gene family during cephalic development in the mouse
Section snippets
Results and discussion
The Tmem16 genes constitute a large, highly conserved family found in all eukaryotes. The number of homologues in a given species seems to increase in relation to evolutionary complexity (Galindo and Vacquier, 2005). In humans and mice, 10 family members have been identified: TMEM16A/Tmem16a through TMEM16H/Tmem16h and TP53I5/Tp53i5 (Katoh and Katoh, 2004a, Katoh and Katoh, 2004b, Katoh and Katoh, 2004c, Katoh and Katoh, 2005, Galindo and Vacquier, 2005) as well as TMEM16K/Tmem16k (Rock et al.,
Experimental procedures
Mouse embryos were collected from time-mated pregnant females. Noon of the day of evidence of a vaginal plug was considered as embryonic day 0.5 (E0.5). Embryos and heads from fetuses and postnatal mice were fixed in 4% paraformaldehyde in PBS and processed for paraffin embedding. Sections were hybridized with 35S-UTP-labelled riboprobes as previously described (Angerer and Angerer, 1992). The probes were as follows: The Tmem16a antisense probe was generated as previously described (Rock et al,
Acknowledgments
This study was supported by the Swedish Research Council-Medicine (Grants 2789, 4567 and 15181), the Thuréus and the Åke Wiberg Foundations, and the Institute of Odontology to A.G.L and A.L. B.D.H. was supported with start-up funds from the University of Florida College of Medicine.
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