Journal of Molecular Biology
Volume 418, Issues 3–4, 4 May 2012, Pages 134-144
Engineering and Structural Characterization of a Linear Polyubiquitin-Specific Antibody
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open access
Graphical Abstract
Highlights
► A linear polyUb antibody with exquisite specificity was engineered. ► The antibody recognizes a conformational epitope unique to linear-linked chains. ► Linear chains are up-regulated upon tumor necrosis factor α stimulation of cells.
Abbreviations used
NFκB
nuclear factor κB
LUBAC
linear Ub chain assembly complex
IF
immunofluorescence
IP
immunoprecipitation
CDR
complementarity-determining region
AQUA
absolute quantification
TNF
tumor necrosis factor
PDB
Protein Data Bank
PBS
phosphate-buffered saline
EDTA
ethylenediaminetetraacetic acid
Keywords
ubiquitin
linkage
phage display
immunoglobulin
X-ray crystallography
Cited by (0)
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Present address: M. L. Matsumoto, Sutro Biopharma, Inc., 310 Utah Avenue, Suite 150, South San Francisco, CA 94080, USA.
Copyright © 2011 Published by Elsevier Ltd.