NeuroanatomyLesional accumulation of P2X4 receptor+ macrophages in rat CNS during experimental autoimmune encephalomyelitis
Section snippets
Rat tissue libraries
Tissue libraries of normal and pathological rat brains have been described before (Schluesener, 1996). Briefly, EAE was induced in Lewis rats (120–160g, Charles River Laboratories, Sulzfeld, Germany) by immunization with the synthetic peptide gpMBP68-84 (YGSLPQKSQRSQDENPV). Development of neurological signs in EAE was scored as follows: 0, no clinical signs; 1, loss of tail tone (flaccid tail); 2, tail weakness plus hindlimb paresis (ataxia); 3, moderate hindlimb paralysis; 4, tetraparesis; and
Results
In the present study, the immunohistochemical distribution of P2X4R was studied in brain and spinal cord tissues of normal rats and animals with EAE.
Discussion
In the present study, we demonstrated that P2X4R was expressed by infiltrating macrophages in the brain and the spinal cord from the early, asymptomatic phase to the recovery phase of EAE. The kinetics of accumulation of P2X4R+ macrophages paralleled those of the recruitment of infiltrate monocytes and the disease severity.
P2X4R, the ATP-gated ion channel, is involved in synaptic transmission in central and peripheral neurons. It has excitatory effects in response to binding of extracellular
Acknowledgments
We thank Ms Katrin Trautmann for technical help. This work has been supported by the DFG (Deutsche Forschungsgemeinschaft).
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