Behavioural NeuroscienceResearch PaperReplacement with GABAergic steroid precursors restores the acute ethanol withdrawal profile in adrenalectomy/gonadectomy mice
Section snippets
Subjects
Drug naive D2 male and female mice were purchased from Jackson West Laboratories (Davis, CA, USA) and were 8–12 weeks old at the time of experiment. Animals were group housed (four/cage, separated by sex) and were allowed free access to rodent chow (Labdiet 5001 rodent diet; PMI International, Richmond, IN, USA) and water. Mice were maintained on a 12 h (6 am to 6 pm) light/dark cycle in polycarbonate cages (Thorens, Hazleton, PA, USA) in a room kept at 21±2 °C with humidity control. Mice were
Results
A multifactorial repeated measures ANOVA was used in order to determine if FIN administration had suppressed ALLO levels during the withdrawal period. Time was a within subjects factor, while sex and drug administration were between subject factors. There were a significant effects of sex [F(1,107)=12.99, P<0.001; female>male] and drug administration [F(1,107)=49.27, P<0.001; VEH>FIN], and no other interactions. These results confirmed that FIN pretreatment had suppressed ALLO production during
Discussion
Previous work in our laboratory has shown that removing the main peripheral sources of PROG- and DOC-derived NAS increased acute EtOH withdrawal severity in D2 male and female mice (Gililland and Finn, 2007). These studies replicated these results as animals that had undergone ADX/GDX surgery had a more severe withdrawal profile than animals that had undergone a SHAM surgery. Further, the lack of effect of GDX on withdrawal severity in D2 male mice suggested that testosterone-derived NAS had no
Acknowledgments
The work presented was supported by grants from the Department of Veterans Affairs and the NIAAA (AA12439 to DAF and AA10760, Portland Alcohol Research Center component to DAF, and an F31 pre-doctoral training grant to KRK, AA017019).
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