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ATP induces nucleotide permeability in rat mast cells

Abstract

ATP IS one of several agonists which stimulate Ca2+-dependent exocytotic degranulation of mast cells, causing the release of histamine and other mediators of immediate hypersensitivity1–3. The concentration–effect relationship of ATP on mast cells is characterised by the sharp onset of self-inhibition as the concentration is raised above the optimum for histamine secretion4. We have previously shown that stimulation of mast cells with IgE-directed ligands (antigen, concanavalin A), chymotrypsin and compound 48/80 is accompanied by an increased turnover of phosphatidylinositol5 similar to that observed for Ca2+-mediated responses in other tissues6. At concentrations of ATP which cause inhibition we have observed a reduction in phosphatidylinositol turnover to less than control levels (unpublished results). We show here that the inhibition by ATP is correlated with an extensive leakage of phosphorylated metabolites from the cells.

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COCKCROFT, S., GOMPERTS, B. ATP induces nucleotide permeability in rat mast cells. Nature 279, 541–542 (1979). https://doi.org/10.1038/279541a0

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