Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Cloning and expression of an inwardly rectifying ATP-regulated potassium channel

Abstract

A complementary DNA encoding an ATP-regulated potassium channel has been isolated by expression cloning from rat kidney. The predicted 45K protein, which features two potential membrane-spanning helices and a proposed ATP-binding domain, represents a major departure from the basic structural design characteristic of voltage-gated and second messenger-gated ion channels. But the presence of an H5 region, which is likely to form the ion conduction pathway, indicates that the protein may share a common origin with voltage-gated potassium channel proteins.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Miller, C. Science 252, 1092–1096 (1991).

    ADS  CAS  PubMed  Google Scholar 

  2. Papazian, D. M., Schwarz, T. L., Tempel, B. L., Jan, Y. N. & Jan, L. Y. Science 237, 749–753 (1987).

    ADS  CAS  PubMed  Google Scholar 

  3. Tempel, B. L., Papazian, D. M., Schwarz, T. L., Jan, Y. N. & Jan, L. Y. Science 237, 770–775 (1987).

    ADS  CAS  PubMed  Google Scholar 

  4. Baumann, A. et al. EMBO J. 6, 3419–3429 (1987).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Kamb, A., Iverson, L. E. & Tanouye, M. A. Cell 50, 405–413 (1987).

    CAS  PubMed  Google Scholar 

  6. Schwarz, T. L., Tempel, B. L., Papazian, D. M., Jan, Y. N. & Jan, L. Y. Nature 331, 137–142 (1988).

    ADS  CAS  PubMed  Google Scholar 

  7. Pongs, O. et al. EMBO J. 7, 1087–1096 (1988).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Kamb, A., Tseng-Crank, J. & Tanouye, M. A. Neuron 1, 421–430 (1988).

    CAS  PubMed  Google Scholar 

  9. Timpe, L. C. et al. Nature 331, 143–145 (1988).

    ADS  CAS  PubMed  Google Scholar 

  10. Iverson, L. E., Tanouye, M. A., Lester, H. A., Davidson, N. & Rudy, B. Proc. natn. Acad. Sci. U.S.A. 85, 5723–5727 (1988).

    ADS  CAS  Google Scholar 

  11. Butler, A., Wei, A., Baker, K. & Salkoff, L. Science 243, 943–947 (1989).

    ADS  CAS  PubMed  Google Scholar 

  12. Wei, A. et al. Science 248, 599–603 (1990).

    ADS  CAS  PubMed  Google Scholar 

  13. Tempel, B. L., Jan, Y. N. & Jan, L. Y. Nature 332, 837–839 (1988).

    ADS  CAS  PubMed  Google Scholar 

  14. Baumann, A., Grupe, A., Ackermann, A. & Pongs, O. EMBO J. 7, 2457–2463 (1988).

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Frech, G. C., VanDongen, A. M. J., Schuster, G., Brown A. M. & Joho, R. H. Nature 340, 642–645 (1989).

    ADS  CAS  PubMed  Google Scholar 

  16. Yokoyama, S. et al. FEBS Lett. 259, 37–42 (1989).

    CAS  PubMed  Google Scholar 

  17. Christie, M. J., Adelman, J. P., Douglass, J. & North, R. A. Science 244, 221–224 (1989).

    ADS  CAS  PubMed  Google Scholar 

  18. Stühmer, W. et al. EMBO J. 8, 3235–3244 (1989).

    PubMed  PubMed Central  Google Scholar 

  19. Swanson, R. et al. Neuron 4, 929–939 (1990).

    CAS  PubMed  Google Scholar 

  20. Pak, M. D. et al. Proc. natn. Acad. Sci. U.S.A. 88, 4386–4390 (1991).

    ADS  CAS  Google Scholar 

  21. Chandy, K. G. et al. Science 247, 973–975 (1990).

    ADS  CAS  PubMed  Google Scholar 

  22. Luneau, C. J. et al. Proc. natn. Acad. Sci. U.S.A. 88, 3932–3936 (1991).

    ADS  CAS  Google Scholar 

  23. Sentenac, H. et al. Science 256, 663–665 (1992).

    ADS  CAS  PubMed  Google Scholar 

  24. Anderson, J. A., Huprikar, S. S., Kochian, L. V., Lucas, W. J. & Gaber, R. F. Proc. natn. Acad. Sci. U.S.A. 89, 3736–3740 (1992).

    ADS  CAS  Google Scholar 

  25. Jan, L. Y. & Jan, Y. N. Cell 69, 715–718 (1992).

    CAS  PubMed  Google Scholar 

  26. Jan, L. Y. & Jan, Y. N. Nature 345, 672 (1990).

    ADS  CAS  PubMed  Google Scholar 

  27. Catterall, W. A. Science 242, 50–61 (1988).

    ADS  CAS  PubMed  Google Scholar 

  28. Hille, B. Ionic Channels of Excitable Membranes 2nd edn (Sinauer, Sunderland, Massachusetts, 1992).

    Google Scholar 

  29. Kaupp, U. B. et al. Nature 342, 762–766 (1989).

    ADS  CAS  PubMed  Google Scholar 

  30. Dhallan, R. S., Yau, K., Schrader, K. A. & Reed, R. R. Nature 347, 184–187 (1990).

    ADS  CAS  PubMed  Google Scholar 

  31. Atkinson, N. S., Robertson, G. A. & Ganetzky, B. Science 253, 551–555 (1991).

    ADS  CAS  PubMed  Google Scholar 

  32. Adelmann, J. P. et al. Neuron 9, 209–216 (1992).

    Google Scholar 

  33. Takumi, T., Ohkubo, H. & Nakanishi, S. Science 242, 1042–1045 (1988).

    ADS  CAS  PubMed  Google Scholar 

  34. Hagiwara, S. & Takahashi, K. J. membr. Biol. 18, 61–80 (1974).

    CAS  PubMed  Google Scholar 

  35. Trube, G. & Hescheler, J. Pflügers Arch. Eur. J. Physiol. 401, 178–184 (1984).

    CAS  Google Scholar 

  36. Belles, B., Malécot, C. O., Hescheler, J. & Trautwein, W. Pflügers Arch. Eur. J. Physiol. 411, 353–360 (1988).

    CAS  Google Scholar 

  37. Bezanilla, F., Caputo, C., Dipolo, R. & Rojas, H. Proc. natn. Acad. Sci. U.S.A. 83, 2743–2745 (1986).

    ADS  CAS  Google Scholar 

  38. Ashcroft, S. J. H. & Ashcroft, F. M. Cellular Signalling 2, 197–214 (1990).

    CAS  PubMed  Google Scholar 

  39. Ashcroft, F. M. A. Rev. Neurosci. 11, 97–118 (1988).

    CAS  Google Scholar 

  40. Wang, W., Sackin, H. & Giebisch, G. A. Rev. Physiol. 54, 81–96 (1992).

    CAS  Google Scholar 

  41. Wang, W. & Giebisch, G. J. gen. Physiol. 98, 35–61 (1991).

    CAS  PubMed  Google Scholar 

  42. Kozak, M. J. biol. Chem. 266, 19867–19870 (1991).

    CAS  PubMed  Google Scholar 

  43. Manley, J. L. Biochim. biophys. Acta 950, 1–12 (1988).

    CAS  PubMed  Google Scholar 

  44. Yellen, G., Jurman, M. E., Abramson, T. & MacKinnon, R. Science 251, 939–942 (1991).

    ADS  CAS  PubMed  Google Scholar 

  45. MacKinnon, R. & Miller, C. Science 245, 1382–1385 (1989).

    ADS  CAS  PubMed  Google Scholar 

  46. MacKinnon, R. & Yellen, G. Science 250, 276–279 (1990).

    ADS  CAS  PubMed  Google Scholar 

  47. Hartmann, H. A. et al. Science 251, 942–944 (1991).

    ADS  CAS  PubMed  Google Scholar 

  48. Yool, A. J. & Schwarz, T. L. Nature 349, 700–704 (1991).

    ADS  CAS  PubMed  Google Scholar 

  49. Heginbotham, L., Abramson, T. & MacKinnon, R. Science 258, 1152–1155 (1992).

    ADS  CAS  PubMed  Google Scholar 

  50. MacKinnon, R., Heginbotham, L. & Abramson, T. Neuron 5, 767–771 (1990).

    CAS  PubMed  Google Scholar 

  51. Kirsch, G. E. et al. Biophys. J. 62, 136–144 (1992).

    ADS  CAS  PubMed  PubMed Central  Google Scholar 

  52. Warmke, J., Drysdale, R. & Ganetzky, B. Science 252, 1560–1562 (1991).

    ADS  CAS  PubMed  Google Scholar 

  53. Kavanaugh, M. P. et al. J. biol. Chem. 266, 7583–7587 (1991).

    CAS  PubMed  Google Scholar 

  54. Heginbotham, L. & MacKinnon, R. Neuron 8, 483–491 (1992).

    CAS  PubMed  Google Scholar 

  55. Kirsch, G. E. et al. Neuron 8, 499–505 (1992).

    CAS  PubMed  Google Scholar 

  56. Papazian, D. M., Timpe, L. C., Jan, Y. N. & Jan, L. Y. Nature 349, 305–310 (1991).

    ADS  CAS  PubMed  Google Scholar 

  57. McCormack, K. et al. Proc. natn. Acad. Sci. U.S.A. 88, 2931–2935 (1991).

    ADS  CAS  Google Scholar 

  58. Walker, J. E., Saraste, M., Runswick, M. J. & Gay, N. J. EMBO J. 1, 945–951 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  59. Saraste, M., Sibbald, P. R. & Wittinghofer, A. Trends biochem. Sci. 15, 430–434 (1990).

    PubMed  Google Scholar 

  60. Kraus, M. H., Issing, W., Miki, T., Popescu, N. C. & Aaronson, S. A. Proc. natn. Acad. Sci. U.S.A. 86, 9193–9197 (1989).

    ADS  CAS  Google Scholar 

  61. Hanks, S. K., Quinn, A. M. & Hunter, T. Science 241, 42–52 (1988).

    ADS  CAS  PubMed  Google Scholar 

  62. Pearson, R. B. & Kemp, B. E. Meth. Enzym. 200, 62–81 (1991).

    CAS  PubMed  Google Scholar 

  63. Rossie, S., Gordon, D. & Catterall, W. A. J. biol. Chem. 262, 17530–17535 (1987).

    CAS  PubMed  Google Scholar 

  64. Swope, S. L., Moss, S. J., Blackstone, C. D. & Huganir, R. L. FASEB J. 6, 2514–2523 (1992).

    CAS  PubMed  Google Scholar 

  65. Riordan, J. R. et al. Science 245, 1066–1073 (1989).

    ADS  CAS  PubMed  Google Scholar 

  66. Hyde, S. C. et al. Nature 346, 362–365 (1990).

    ADS  CAS  PubMed  Google Scholar 

  67. MacKinnon, R. Nature 350, 232–235 (1991).

    ADS  CAS  PubMed  Google Scholar 

  68. Liman, E. R., Tytgat, J. & Hess, P. Neuron 9, 861–871 (1992).

    CAS  PubMed  Google Scholar 

  69. Durell, S. R. & Guy, H. R. Biophys. J. 62, 238–250 (1992).

    ADS  CAS  PubMed  PubMed Central  Google Scholar 

  70. Hartmann, E., Rapoport, T. A. & Lodish, H. F. Proc. natn. Acad. Sci. U.S.A. 86, 5786–5790 (1989).

    ADS  CAS  Google Scholar 

  71. Dalbey, R. E. Trends biochem. Sci. 15, 253–257 (1990).

    CAS  PubMed  Google Scholar 

  72. Takano, M., Qin, D. & Noma, A. Am. J. Physiol. 258, H45–H50 (1990).

    CAS  PubMed  Google Scholar 

  73. Augustine, C. K. & Bezanilla, F. J. gen. Physiol. 95, 245–271 (1990).

    CAS  PubMed  Google Scholar 

  74. Perozo, E., Vandenberg, C. A., Jong, D. S. & Bezanilla, F. J. gen. Physiol. 98, 1–17 (1991).

    CAS  PubMed  Google Scholar 

  75. Ohno-Shosaku, T., Zünkler, B. J. & Trube, G. Pflügers Arch. Eur. J. Physiol. 408, 133–138 (1987).

    CAS  Google Scholar 

  76. Stelzer, A., Kay, A. R. & Wong, R. K. S. Science 241, 339–341 (1988).

    ADS  CAS  PubMed  Google Scholar 

  77. Chung, S., Reinhart, P. H., Martin, B. L., Brautigan, D. & Levitan, I. B. Science 253, 560–562 (1991).

    ADS  CAS  PubMed  Google Scholar 

  78. Kim, D. J. Physiol. 437, 133–155 (1991).

    CAS  PubMed  PubMed Central  Google Scholar 

  79. Frace, A. M., Maruoka, F. & Noma, A. Pflügers Arch. Eur. J. Physiol. 421, 94–96 (1992).

    Google Scholar 

  80. West, J. W., Numann, R., Murphy, B. J., Scheuer, T. & Catterall, W. A. Science 254, 866–868 (1991).

    ADS  CAS  PubMed  Google Scholar 

  81. Chang, C. F., Gutierrez, L. M., Mundina-Weilenmann, C. & Hosey, M. M. J. biol. Chem. 266, 16395–16400 (1991).

    CAS  PubMed  Google Scholar 

  82. Rehm, H. et al. Biochemistry 28, 6455–6460 (1989).

    CAS  PubMed  Google Scholar 

  83. Moss, S. J., Smart, T. G., Blackstone, C. D. & Huganir, R. L. Science 257, 661–665 (1992).

    ADS  CAS  PubMed  Google Scholar 

  84. Anderson, M. P. et al. Cell 67, 775–784 (1991).

    CAS  PubMed  Google Scholar 

  85. Rich, D. P. et al. Science 253, 205–207 (1991).

    ADS  CAS  PubMed  Google Scholar 

  86. Cheng, S. H. et al. Cell 66, 1027–1036 (1991).

    CAS  PubMed  Google Scholar 

  87. Anderson, M. P. & Welsh, M. J. Science 257, 1701–1704 (1992).

    ADS  CAS  PubMed  Google Scholar 

  88. Hoshi, T., Zagotta, W. N. & Aldrich, R. W. Science 250, 533–538 (1990).

    ADS  CAS  PubMed  Google Scholar 

  89. Stühmer, W. et al. Nature 339, 597–603 (1989).

    ADS  PubMed  Google Scholar 

  90. Armstrong, C. M. & Bezanilla, F. J. gen. Physiol. 70, 567–590 (1977).

    CAS  PubMed  Google Scholar 

  91. Tabcharani, J. A., Chang, X.-B., Riordan, J. R. & Hanrahan, J. W. Nature 352, 628–631 (1991).

    ADS  CAS  PubMed  Google Scholar 

  92. Hebert, S. C. & Andreoli, T. E. J. membr. Biol. 80, 221–233 (1984).

    CAS  PubMed  Google Scholar 

  93. Maniatis, T., Fritsch, E. & Sambrook, J. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  94. Gubler, U. & Hoffman, B. J. Gene 25, 263–269 (1983).

    CAS  PubMed  Google Scholar 

  95. Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Pflügers Arch. Eur. J. Physiol. 391, 85–100 (1981).

    CAS  Google Scholar 

  96. Methfessel, C. et al. Pflügers Arch. Eur. J. Physiol. 407, 577–588 (1986).

    CAS  Google Scholar 

  97. Lederer, W. J. & Nichols, C. G. J. Physiol. 419, 193–211 (1989).

    CAS  PubMed  PubMed Central  Google Scholar 

  98. Lopatin, A. N. & Nichols, C. G. J. Pharmac. exp. Ther. (in the press).

  99. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    ADS  CAS  Google Scholar 

  100. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).

    CAS  PubMed  Google Scholar 

  101. Noda, M. et al. Nature 320, 188–192 (1986).

    ADS  CAS  PubMed  Google Scholar 

  102. Hanks, S. K. Curr. Opin. struc. Biol. 1, 369–383 (1991).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ho, K., Nichols, C., Lederer, W. et al. Cloning and expression of an inwardly rectifying ATP-regulated potassium channel. Nature 362, 31–38 (1993). https://doi.org/10.1038/362031a0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/362031a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing