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Direct stimulation of Jak/STAT pathway by the angiotensin II AT1 receptor

Nature volume 375, pages 247–250 (1995)Cite this article

Abstract

THE peptide angiotensin II is the effector molecule of the renin-angiotensin system. All the haemodynamic effects of angiotensin II, including vasoconstriction and adrenal aldosterone release, are mediated through a single class of cell-surface receptors known as AT1 (refs 1, 2). These receptors contain the structural features of the G-protein-coupled receptor superfamily3. We show here that angiotensin II induces the rapid phosphorylation of tyrosine in the intracellular kinases Jak2 and Tyk2 in rat aortic smooth-muscle cells and that this phosphorylation is associated with increased activity of Jak2. The Jak family substrates STAT1 and STAT2 (for signal transducers and activators of transcription) are rapidly tyrosine-phosphorylated in response to angiotensin II. We also find that Jak2 co-precipitates with the AT1, receptor, indicating that G-protein-coupled receptors may be able to signal through the intracellular phosphorylation pathways used by cytokine receptors4,5.

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, Division of Cardiology, Emory University, Atlanta, Georgia, 30322, USA

    Mario B. Marrero, Bernhard Schieffer, William G. Paxton & Kenneth E. Bernstein

  2. Department of Medicine, Division of Cardiology, Emory University, Atlanta, Georgia, 30322, USA

    Patrick Delafontaine

  3. Santa Cruz Biotechnology Inc., Santa Cruz, California, 95060, USA

    Lauri Heerdt

  4. Department of Medicine, Division of Cardiology, University of Washington, Seattle, Washington, 98195, USA

    Bradford C. Berk

Authors
  1. Mario B. Marrero
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  2. Bernhard Schieffer
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  4. Lauri Heerdt
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  5. Bradford C. Berk
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  6. Patrick Delafontaine
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  7. Kenneth E. Bernstein
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Marrero, M., Schieffer, B., Paxton, W. et al. Direct stimulation of Jak/STAT pathway by the angiotensin II AT1 receptor. Nature 375, 247–250 (1995). https://doi.org/10.1038/375247a0

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