Elsevier

Kidney International

Volume 79, Issue 11, 1 June 2011, Pages 1228-1235
Kidney International

Original Article
The possible role of ChemR23/Chemerin axis in the recruitment of dendritic cells in lupus nephritis

https://doi.org/10.1038/ki.2011.32Get rights and content
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Dendritic cells (DCs) have a pivotal role in the autoimmune response of systemic lupus erythematosus. Plasmacytoid DCs infiltrate the kidney of patients with lupus nephritis, but factors regulating their recruitment to the kidney are unknown. Chemerin is the recently identified natural ligand of ChemR23, a receptor highly expressed by plasmacytoid DCs. We performed immunohistochemical and immunofluorescence analysis to study the ChemR23/Chemerin axis in renal biopsies from patients with lupus nephritis. We found ChemR23-positive DCs had infiltrated the kidney tubulointerstitium in patients with severe lupus nephritis. Chemerin association with tubular epithelial cells and renal lymphatic endothelial cells was found in patients with lupus nephritis but not in normal kidneys. Proximal tubular epithelial cells produced Chemerin in vitro, which was significantly down-modulated by added tumor necrosis factor (TNF)-α and interferon-γ as measured by quantitative PCR and enzyme-linked immunosorbent assay. Interestingly, TNF-α was capable of inducing a functionally active form of renal Chemerin, resulting in an efficient transendothelial migration of plasmacytoid DCs measured in transwell systems. Thus, the ChemR23/Chemerin axis may have a role in the recruitment of DCs within the kidney in patients affected by lupus nephritis.

KEYWORDS

chemokine
chemokine receptor
lupus nephritis
systemic lupus erythematosus

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The authors have no financial disclosure to make relevant to the subject matter of this study.

This study was partially supported by MIUR (Ministero dell’Istruzione Università e Ricerca) and Istituto Superiore di Sanità (ISS); GDP was supported by a Ph.D. fellowship from the Doctorate in Biotechnologies applied to organ and tissue transplantation, University of Bari.

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The authors equally contributed to the present study.