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The transmembrane receptor protein tyrosine phosphatase DEP1 interacts with p120ctn

Oncogene volume 21, pages 7067–7076 (2002)Cite this article

Abstract

The receptor-like protein tyrosine phosphatase DEP1, also known as CD148, is expressed predominantly in epithelial cells, in a variety of tumor cell lines, and in lymphocytes. Expression of DEP1 is enhanced at high cell density, and this observation suggests that DEP1 may function in the regulation of cell adhesion and possibly contact inhibition of cell growth. In order to investigate the function of DEP1, substrate-trapping mutants of the phosphatase were used to identify potential substrates. GST-fusion proteins containing the DEP1 catalytic domain with a substrate-trapping D/A mutation were found to interact with p120ctn, a component of adherens junctions. DEP1 also interacted with other members of the catenin gene family including β-catenin and γ-catenin. The interaction with p120ctn is likely to be direct, as the interaction occurs in K562 cells lacking functional adherens junctions and E-cadherin expression. Catalytic domains of the tyrosine phosphatases PTP-PEST, CD45, and PTPβ did not interact with proteins of the catenin family to detectable levels, suggesting that the interaction of DEP1 with these proteins is specific. DEP1 expression was concentrated at sites of cell–cell contact in A549 cells. p120ctn was found to colocalize with these structures. Together these data suggest an important role for DEP-1 in the function of cell–cell contacts and adherens junctions.

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Acknowledgements

We thank Antoni Gaya and Eduard Palou for their kind gift of DEP1 143-41 antibody, and David Rimm for the p120ctn cDNA clone. We gratefully acknowledge Ron Hill, David Markby, and Audie Rice for comments on the manuscript.

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  1. SUGEN Inc., 230 East Grand Avenue, South San Francisco, 94080, California, CA, USA

    Leslie J Holsinger, Kevin Ward, Bill Duffield, Joseph Zachwieja & Bahija Jallal

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  1. Leslie J Holsinger
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  2. Kevin Ward
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Correspondence to Leslie J Holsinger.

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Holsinger, L., Ward, K., Duffield, B. et al. The transmembrane receptor protein tyrosine phosphatase DEP1 interacts with p120ctn. Oncogene 21, 7067–7076 (2002). https://doi.org/10.1038/sj.onc.1205858

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