Basic—Liver, Pancreas, and Biliary TractThe Hepatic Vagus Nerve Attenuates Fas-Induced Apoptosis in the Mouse Liver via α7 Nicotinic Acetylcholine Receptor
Section snippets
Animals
Male C57BL/6 (B6) mice (8 weeks of age; Charles River Japan, Shizuoka, Japan) were kept on a 12-hour light/12-hour dark cycle (room temperature, 23°C–25°C) with food and water freely available. This experiment was reviewed by the Ethics Committee on Animal Experiments of the Graduate School of Medical Sciences, Kyushu University, and was carried out under the control of the Guidelines for Animal Experiments of the Graduate School of Medical Sciences, Kyushu University, and Law (No. 105) and
Validity of Selective Hepatic Vagotomy
All mice were alive after selective denervation of the hepatic vagus nerve, and food intake, weight gain, and behavior in the vagotomized mice were not different from those in the sham-operated mice. There was no significant difference in the norepinephrine level between the sham-operated and vagotomized livers (50.1 ± 8.40 and 64.2 ± 15.6 ng/g tissue, respectively, n = 10 per group). The urine densities in the vagotomized mice were significantly lower than those in the sham-operated mice at 18
Discussion
In this study, we found that hepatic vagus nerve denervation exaggerated Fas-induced fulminant hepatitis. Supplementation with nicotine and PNU-282987, an α7 nicotinic AChR agonist, completely inhibited such an exaggeration, although these supplementations failed to affect in vitro Fas-induced hepatocyte apoptosis. Furthermore, the accelerating effect of the hepatic vagotomy was completely suppressed by the elimination of Kupffer cells. These findings indicate that the hepatic vagus nerve
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2019, Molecular MetabolismCitation Excerpt :Accumulating evidence has demonstrated that the vagus nerve plays a crucial role in the brain-liver interaction [46]. The vagus nerve is a mixed nerve composed of 20% efferent and 80% afferent fibers [46]. Previous studies also reported that hepatic peroxisome proliferators-activated receptor γ (PPAR-γ) and glucokinase affects SNS activity through the afferent vagal signals originating in the liver [6,47].
Nicotinic acetylcholine receptor α7 subunit improves energy homeostasis and inhibits inflammation in nonalcoholic fatty liver disease
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Supported by Grants-in-aid for General Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology, Japan (No. 16390200, No. 17390210, No. 17659205, and No. 19390192); the Smoking Research Foundation (Japan); the Sumitomo Life Social Welfare Services Foundation (Japan); the Kanae Foundation for the Promotion of Medical Science (Japan); and the British Heart Foundation (United Kingdom).
Conflicts of interest and financial disclosures: All the authors declare that no potential competing interests exist.