Novel capsaicin (VR1) and purinergic (P2X3) receptors in Hirschsprung's intestine☆
Section snippets
HSCR and control patients
Full-thickness, surgically resected colonic tissues were obtained from 8 patients with approval from the local ethics committee. All patients had sporadic distal colonic or short-segment-disease without clinical evidence of an established major genetic syndrome, eg, Down's syndrome/Shah-Waardenburg syndrome (6 male, 2 female, age 0.2 to 1.0 years). Specimens were taken from aganglionic and normoganglionic regions on the basis of histologic characterization. Age-matched control tissues were
VR1
In control tissues, VR1 immunoreactivity was detected in nerve fascicles in the myenteric plexus with fibers in the muscle layers (Fig 1a).
Discussion
The basic neural reflex circuit for normal peristalsis consists of sensory neurons, interneurons and motor neurons. Afferent nerves carry impulses generated by bowel wall distension and chemical or mechanical irritation of bowel mucosa to activate interneurons that stimulate motor excitatory neurons proximally and inhibitory neurons distally.16 VR1 and P2X3 are novel receptors of subsets of nociceptive sensory neurons. Our finding of VR1-and P2X3-immunoreactive nerves in the myenteric and
References (26)
- et al.
Molecular characterization of the Drosophila trp locus: A putative integral membrane protein required for phototransduction
Neuron
(1989) - et al.
Observations on the algogenic actions of adenosine compounds on human blister base preparation
Pain
(1977) - et al.
A quantitative study of acetylcholine in Hirschsprung's disease
J Pediatr Surg
(1980) - et al.
Origin, course and endings of abnormal enteric nerve fibres in Hirschsprung's disease defined by whole-mount immunohistochemistry
J Pediatr Surg
(1990) - et al.
Quantitative analysis of neuronal innervation in the aganglionic bowel of patients with Hirschsprung's disease
J Pediatr Surg
(1995) - et al.
Increased capsaicin receptor VR1immunoreactivity in inflamed human bowel
Lancet
(2001) - et al.
The glucose oxidase-DAB-nickel method in peroxidase histochemistry of the nervous system
Neuroscience Lett
(1988) - et al.
New insights into peptidergic abnormalities in Hirschsprung's disease by whole-mount immunohistochemistry
J Pediatr Surg
(1991) - et al.
NPY hyperinnervation in Hirschsprung's disease: Both adrenergic and nonadrenergic fibers contribute
J Pediatr Surg
(1991) - et al.
Glial-derived neurotrophic factor in human adult and fetal intestine and in Hirschsprung's disease
Gastroenterology
(1997)
Mast cells and gut nerve development: Implications for Hirschsprung's disease and intestinal neuronal dysplasia
J Pediatr Surg
The capsaicin receptor: A heat-activated ion channel in the pain pathway
Nature
ATP (P2X) receptors and pain
Cited by (0)
- ☆
Address reprint requests to Professor Praveen Anand, Peripheral Neuropathy Unit, Imperial College School of Medicine, Hammersmith Hospital, London, England, or Professor Paul K.H. Tam, Department of Surgery, University of Hong Kong Medical Center, Queen Mary Hospital, Hong Kong SAR, China.