Chest
Original ResearchObstructive Lung DiseasesElevated Airway Purines in COPD
Section snippets
Study Subjects
Subjects were participants in one of two observational studies: RES19044 (A Multi-center Cohort Study to Evaluate Radiological, Physiological and Biochemical Biomarkers in Patients with Chronic Obstructive Pulmonary Disease and Age and Gender Matched Controls)18 or ECLIPSE (Evaluation of COPD Longitudinally to Identify Predictive Surrogate End-points) (clinicaltrials.gov; Identifier: NCT00292552).19 RES19044 was approved by Institutional Review Boards at St. Elizabeth's Medical Center (00195)
Adenosine and Other Biomarkers Measured in EBC
Airway concentrations of purines and other metabolites were successfully measured in EBC from 153 subjects: 36 healthy nonsmokers (NS group), 28 healthy smokers (S group), and 89 subjects with COPD (GOLD stage II, 29; GOLD stage III, 29; and GOLD stage IV, 31). Age and gender distribution was generally similar among cohorts, whereas FEV1 was decreased significantly in COPD (Table 1). Use of inhaled corticosteroids was common in the COPD cohorts (Table 1), as was use of inhaled long-acting β
Discussion
This study demonstrated that purine concentrations on airway surfaces, as measured through analysis of EBC, are elevated in COPD and correlated with markers of disease severity. These results are consistent with previous human4, 5 and animal studies9, 13 that link adenosine and airway inflammation and suggest that this purine plays a pathophysiologic role in COPD.10, 23, 24 Although indirect evidence of elevated airway adenosine in COPD has previously been reported,11 this study represents the
Acknowledgments
Author contributions: Dr Esther had full access to the data and vouches for the integrity of the manuscript.
Dr Esther: contributed to performing mass spectrometry, data analyses, and wrote and critically reviewed the manuscript.
Dr Lazaar: contributed to providing samples and critically reviewed the manuscript.
Dr Bordonali: contributed to providing biostatistical support and critically reviewed the manuscript.
Dr Qaqish: contributed to providing biostatistical support and critically reviewed the
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Cited by (0)
Funding/Support: This study was supported by the National Institutes of Health [Grants 1K23HL089708, SCCOR HL34322 MTCC] and GlaxoSmithKline.
Reproduction of this article is prohibited without written permission from the American College of Chest Physicians (http://www.chestpubs.org/site/misc/reprints.xhtml).