Abstract
Deposition of beta-amyloid (A beta) in the brain is considered to be one of the most critical events in the onset of Alzheimer's disease (AD). In order to identify factors involved in the exacerbation of AD, we investigated transcriptionally A beta-induced genes using a cDNA subtraction technique in rat astrocytes. One gene obtained was rat prostaglandin (PG) E synthase. In this report, we present the deduced sequence for rat PGE synthase for the first time and demonstrate the induction of PGE synthase mRNA by treatment of cells with A beta. Our results suggest a possibly significant role of this enzyme in the progression of AD.
MeSH terms
-
Alzheimer Disease / enzymology
-
Amino Acid Sequence
-
Amyloid beta-Peptides / pharmacology*
-
Animals
-
Astrocytes / drug effects
-
Astrocytes / enzymology*
-
Cells, Cultured
-
Enzyme Induction / drug effects
-
Enzyme Induction / genetics
-
Intramolecular Oxidoreductases / biosynthesis*
-
Intramolecular Oxidoreductases / chemistry
-
Intramolecular Oxidoreductases / genetics*
-
Molecular Sequence Data
-
Organ Specificity / genetics
-
Prostaglandin-E Synthases
-
RNA, Messenger / biosynthesis*
-
Rats
-
Rats, Wistar
-
Sequence Homology, Amino Acid
Substances
-
Amyloid beta-Peptides
-
RNA, Messenger
-
Intramolecular Oxidoreductases
-
Prostaglandin-E Synthases