Abstract
High-dose administration of cocaine or methamphetamine to rats acutely (< or = 24 h) alters vesicular dopamine transport. This study elucidates the nature of these changes. Results reveal a differential redistribution of the vesicular monoamine transporter-2 (VMAT-2) within striatal synaptic terminals after drug treatment. In particular, cocaine shifts VMAT-2 protein from a synaptosomal membrane fraction to a vesicle-enriched fraction, as assessed ex vivo in fractions prepared from treated rats. In contrast, methamphetamine treatment redistributes VMAT-2 from a vesicle-enriched fraction to a location that is not retained in a synaptosomal preparation. These data suggest that psychostimulants acutely and differentially affect VMAT-2 subcellular localization.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Central Nervous System Stimulants / pharmacology*
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Cocaine / pharmacology*
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Male
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Membrane Glycoproteins / metabolism*
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Membrane Transport Proteins*
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Methamphetamine / pharmacology*
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Neostriatum / drug effects
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Neostriatum / metabolism
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Neuropeptides*
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Presynaptic Terminals / drug effects
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Presynaptic Terminals / metabolism
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Rats
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Rats, Sprague-Dawley
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Synaptic Vesicles / drug effects
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Synaptic Vesicles / metabolism
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Vesicular Biogenic Amine Transport Proteins
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Vesicular Monoamine Transport Proteins
Substances
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Central Nervous System Stimulants
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Membrane Glycoproteins
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Membrane Transport Proteins
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Neuropeptides
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Vesicular Biogenic Amine Transport Proteins
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Vesicular Monoamine Transport Proteins
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Methamphetamine
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Cocaine