Abstract
The model organism Arabidopsis thaliana contains a restricted set of heterotrimeric G-protein subunits, with only one canonical Galpha subunit (AtGPA1), one Gbeta subunit (AtAGB1), and two Ggamma subunits (AtAGG1 and AtGG2) identified. We have identified a novel additional component of heterotrimeric G-protein signaling in the A. thaliana genome, regulator of G-protein signaling-1 (AtRGS1). This protein has the predicted topology and structure of a G-protein-coupled receptor in that it contains seven transmembrane domains, but AtRGS1 also contains a unique C-terminal extension, namely a regulator of G-protein signaling domain (RGS box). This article describes methods for the purification and in vitro functional analysis of the RGS box of AtRGS1.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
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Review
MeSH terms
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Amino Acid Sequence
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Arabidopsis / genetics
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Arabidopsis / metabolism*
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Arabidopsis Proteins / chemistry
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / isolation & purification*
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Arabidopsis Proteins / metabolism*
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Conserved Sequence
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Escherichia coli / genetics
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GTP-Binding Protein Regulators
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GTP-Binding Protein alpha Subunits
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GTP-Binding Protein beta Subunits
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GTP-Binding Protein gamma Subunits
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Genome, Plant
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Heterotrimeric GTP-Binding Proteins / chemistry
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Heterotrimeric GTP-Binding Proteins / genetics
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Heterotrimeric GTP-Binding Proteins / metabolism*
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Molecular Sequence Data
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Precipitin Tests
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Protein Structure, Secondary
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Protein Structure, Tertiary
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RGS Proteins / chemistry
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RGS Proteins / genetics
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RGS Proteins / isolation & purification*
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RGS Proteins / metabolism*
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Recombinant Fusion Proteins / metabolism
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Sequence Homology, Amino Acid
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Surface Plasmon Resonance
Substances
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Arabidopsis Proteins
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GTP-Binding Protein Regulators
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GTP-Binding Protein alpha Subunits
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GTP-Binding Protein beta Subunits
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GTP-Binding Protein gamma Subunits
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RGS Proteins
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Recombinant Fusion Proteins
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Heterotrimeric GTP-Binding Proteins