Abstract
Tight junctions (TJ) are multiprotein complexes that function to regulate paracellular transport of molecules through epithelial and endothelial cell layers. Many new tight junction-associated proteins have been identified in the past few years, and their functional roles and interactions have just begun to be elucidated. In this paper, we describe a novel protein LYsine-RIch CEACAM1 co-isolated (LYRIC) that is widely expressed and highly conserved between species. LYRIC has no conserved domains that would indicate function and does not appear to be a member of a larger protein family. Data from analysis of rat and human tissue sections and cell lines show that LYRIC colocalizes with tight junction proteins ZO-1 and occludin in polarized epithelial cells, suggesting that LYRIC is part of the tight junction complex. LYRIC dissociates from ZO-1 when junctional complexes are disrupted, and as tight junctions reform, ZO-1 relocalizes before LYRIC. These results suggest that LYRIC is most likely not a structural component required for TJ formation, but rather is recruited during the maturation of the tight junction complex.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, CD / metabolism
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Antigens, Differentiation / metabolism
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Cadherins / metabolism
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Carrier Proteins / chemistry
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Carrier Proteins / isolation & purification
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Carrier Proteins / metabolism*
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Cell Adhesion / genetics
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Cell Adhesion Molecules
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Cell Line
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Cell Line, Tumor
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Cell Polarity / genetics*
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DNA, Complementary / analysis
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DNA, Complementary / genetics
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Epithelial Cells / metabolism*
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Epithelial Cells / ultrastructure
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Humans
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Male
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Membrane Proteins / genetics
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Membrane Proteins / isolation & purification
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Membrane Proteins / metabolism*
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Microscopy, Electron, Transmission
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Molecular Sequence Data
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Phosphoproteins / metabolism
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RNA-Binding Proteins
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Rats
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Rats, Inbred F344
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Tight Junctions / metabolism*
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Tight Junctions / ultrastructure
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Zonula Occludens-1 Protein
Substances
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Antigens, CD
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Antigens, Differentiation
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CD66 antigens
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Cadherins
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Carrier Proteins
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Cell Adhesion Molecules
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DNA, Complementary
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MTDH protein, human
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Membrane Proteins
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Mtdh protein, rat
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Phosphoproteins
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RNA-Binding Proteins
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TJP1 protein, human
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Tjp1 protein, rat
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Zonula Occludens-1 Protein
Associated data
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GENBANK/AF100421
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GENBANK/AY082966