Cloning by functional expression of platelet-activating factor receptor from guinea-pig lung

Z Honda; M Nakamura; I Miki; M Minami; T Watanabe; Y Seyama; H Okado; H Toh; K Ito; T Miyamoto

doi:10.1038/349342a0

Cloning by functional expression of platelet-activating factor receptor from guinea-pig lung

Nature. 1991 Jan 24;349(6307):342-6. doi: 10.1038/349342a0.

Authors

Z Honda¹, M Nakamura, I Miki, M Minami, T Watanabe, Y Seyama, H Okado, H Toh, K Ito, T Miyamoto, et al.

Affiliation

¹ Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Japan.

PMID: 1846231
DOI: 10.1038/349342a0

Abstract

Platelet-activating factor (PAF), a unique phospholipid mediator, possesses potent proinflammatory, smooth-muscle contractile and hypotensive activities, and appears to be crucial in the pathogenesis of bronchial asthma and in the lethality of endotoxin and anaphylactic shock. Despite this, little is known of the molecular properties of the PAF receptor and related signal transduction systems. Although several lines of evidence suggest that activation of the PAF receptor stimulates phospholipase C and subsequent inositol trisphosphate formation through G protein(s), the PAF receptor and calcium channel are reported to show a close relation. As a first approach to cloning lipid autacoid receptors, we have isolated complementary DNA for the PAF receptors. Our strategy involved gene expression in Xenopus laevis oocytes and electrophysiological detection of PAF-induced responses. Sequence analysis indicates that the receptor belongs to the superfamily of G protein-coupled receptors.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cell Line
Cloning, Molecular*
DNA / genetics
Electrophysiology
GTP-Binding Proteins
Gene Expression*
Guinea Pigs
Lung / chemistry*
Molecular Sequence Data
Oocytes / physiology
Platelet Activating Factor / analogs & derivatives
Platelet Activating Factor / antagonists & inhibitors
Platelet Activating Factor / pharmacology
Platelet Membrane Glycoproteins*
Receptors, Cell Surface / chemistry
Receptors, Cell Surface / genetics*
Receptors, Cell Surface / physiology
Receptors, G-Protein-Coupled*
Sequence Homology, Nucleic Acid
Transfection
Xenopus laevis

Substances

Platelet Activating Factor
Platelet Membrane Glycoproteins
Receptors, Cell Surface
Receptors, G-Protein-Coupled
platelet activating factor receptor
DNA
GTP-Binding Proteins

Associated data

GENBANK/X52371
GENBANK/X52372
GENBANK/X53984
GENBANK/X56736
GENBANK/X57181
GENBANK/X57182
GENBANK/X57183
GENBANK/X57184
GENBANK/X58548
GENBANK/X61719