Abstract
The type 5 G protein beta subunit (Gbeta5) can form complexes with members of the regulator of G protein signaling 7 (RGS7) family, but its relevance to neuronal G protein signaling is unclear. We found that mouse RGS7-Gbeta5 complexes bound to G protein-gated potassium channels and facilitated their functional coupling to GABA(B) receptors in neurons. Our findings identify a compartmentalization mechanism that is critical for ensuring high temporal resolution of neuronal G protein signaling.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Baclofen / pharmacology
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Cell Line
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Cells, Cultured
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G Protein-Coupled Inwardly-Rectifying Potassium Channels / genetics
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G Protein-Coupled Inwardly-Rectifying Potassium Channels / metabolism*
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GABA Agonists / pharmacology
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GTP-Binding Protein beta Subunits / genetics
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GTP-Binding Protein beta Subunits / metabolism*
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Hippocampus / drug effects
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Hippocampus / physiology
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Humans
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Inhibitory Postsynaptic Potentials / drug effects
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Inhibitory Postsynaptic Potentials / physiology
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Membrane Potentials / drug effects
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Membrane Potentials / physiology
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Motor Activity / drug effects
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Motor Activity / physiology
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Neural Inhibition / drug effects
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Neural Inhibition / physiology*
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Neurons / drug effects
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Neurons / physiology*
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RGS Proteins / metabolism*
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Receptors, GABA-B / metabolism
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Synaptic Transmission / drug effects
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Synaptic Transmission / physiology*
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Time Factors
Substances
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G Protein-Coupled Inwardly-Rectifying Potassium Channels
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GABA Agonists
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GTP-Binding Protein beta Subunits
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Gnb5 protein, mouse
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RGS Proteins
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Receptors, GABA-B
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Rgs7 protein, mouse
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Baclofen