Molecular cloning of a novel G protein-coupled receptor that may belong to the neuropeptide receptor family

C Eva; K Keinänen; H Monyer; P Seeburg; R Sprengel

doi:10.1016/0014-5793(90)80377-u

Molecular cloning of a novel G protein-coupled receptor that may belong to the neuropeptide receptor family

FEBS Lett. 1990 Oct 1;271(1-2):81-4. doi: 10.1016/0014-5793(90)80377-u.

Authors

C Eva¹, K Keinänen, H Monyer, P Seeburg, R Sprengel

Affiliation

¹ Zentrum für Molekulare Biologie Heidelberg, University of Heidelberg, FRG.

PMID: 2172008
DOI: 10.1016/0014-5793(90)80377-u

Abstract

A novel cDNA encoding a putative G protein-coupled receptor was isolated from a rat forebrain cDNA library by homology screening. Sequence comparison demonstrates that the encoded polypeptide containing seven transmembrane domains shows highest homology with tachykinin receptors, particularly in the transmembrane domains and the first intracellular loop. The mRNA for this new receptor seems specifically expressed in brain and is prominently localized in specific nuclei of the rat brain, including thalamus, cerebral cortex and hippocampus. We speculate that a neuropeptide may be the natural ligand of this novel G protein-coupled receptor.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Brain Chemistry
Cloning, Molecular
DNA / chemistry
GTP-Binding Proteins / genetics*
Molecular Sequence Data
RNA, Messenger / biosynthesis
Rats
Receptors, Cell Surface / genetics*
Receptors, G-Protein-Coupled*
Receptors, Neuropeptide*
Receptors, Neurotransmitter / genetics*
Receptors, Tachykinin
Sequence Homology, Nucleic Acid

Substances

Npy1r protein, rat
RNA, Messenger
Receptors, Cell Surface
Receptors, G-Protein-Coupled
Receptors, Neuropeptide
Receptors, Neurotransmitter
Receptors, Tachykinin
DNA
GTP-Binding Proteins