Abstract
The expression of the programmed death 1 (PD-1) gene is an indicator of exhausted T-cells with decreased activation and function. It remains unknown, however, whether the methylation status of the PD-1 gene promoter is associated with PD-1 expression level. This study shows the changes of PD-1 expression levels and the demethylation status of the PD-1 promoter region in Molt-4 cells under different concentrations of 5-azacytidine (5-Zac). The result demonstrated that DNA demethylation at PD-1 promoter may contribute to PD-1 overexpression.
Crown Copyright © 2011. Published by Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / drug effects
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Azacitidine / pharmacology*
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Base Sequence
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Cell Line, Tumor
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CpG Islands
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DNA Methylation / drug effects
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DNA Primers / genetics
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DNA, Neoplasm / genetics
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DNA, Neoplasm / metabolism
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Gene Expression / drug effects
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Humans
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Programmed Cell Death 1 Receptor / genetics*
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Promoter Regions, Genetic*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Neoplasm / genetics
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RNA, Neoplasm / metabolism
Substances
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DNA Primers
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DNA, Neoplasm
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PDCD1 protein, human
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Programmed Cell Death 1 Receptor
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RNA, Messenger
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RNA, Neoplasm
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Azacitidine