Abstract
A cDNA clone encoding a transporter for the neurotransmitter gamma-aminobutyric acid in human brain was cloned and sequenced. The cDNA contains an open reading frame encoding a hydrophobic protein of 599 amino acids with a calculated molecular weight of 67022 Da. Hydropathy analysis revealed twelve potential transmembrane segments. The human protein is highly homologous to the protein from rat brain. Northern hybridization demonstrated a ubiquitous distribution of the transporter in various parts of the brain.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Brain / physiology
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Carrier Proteins / genetics
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Cloning, Molecular
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DNA / genetics
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GABA Plasma Membrane Transport Proteins
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Humans
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Membrane Proteins*
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Membrane Transport Proteins*
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics*
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Organic Anion Transporters*
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Restriction Mapping
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gamma-Aminobutyric Acid / metabolism
Substances
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Carrier Proteins
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GABA Plasma Membrane Transport Proteins
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Membrane Proteins
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Membrane Transport Proteins
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Nerve Tissue Proteins
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Organic Anion Transporters
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gamma-Aminobutyric Acid
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DNA