The interaction between the TSH receptor and Graves' sera with TSH agonist or antagonist properties

E D Jones; F A Hashim; F M Creagh; S E Williams; B R Smith

doi:10.1016/0303-7207(85)90030-9

The interaction between the TSH receptor and Graves' sera with TSH agonist or antagonist properties

Mol Cell Endocrinol. 1985 Jul;41(2-3):257-61. doi: 10.1016/0303-7207(85)90030-9.

Authors

E D Jones, F A Hashim, F M Creagh, S E Williams, B R Smith

PMID: 2991045
DOI: 10.1016/0303-7207(85)90030-9

Abstract

The A subunit of the TSH receptor was prepared by reduction of human thyroid membranes with dithiothreitol, and partially purified by gel filtration. The ability of Graves' sera to inhibit TSH binding to the TSH receptor and to stimulate cyclic AMP release from isolated thyroid cells was abolished by incubation with crude and partially purified preparations of the A subunit.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cyclic AMP / biosynthesis
Graves Disease / immunology*
Graves Disease / physiopathology
Humans
Macromolecular Substances
Protein Binding
Receptors, Cell Surface / physiology*
Receptors, Thyrotropin
Thyrotropin / antagonists & inhibitors
Thyrotropin / physiology*

Substances

Macromolecular Substances
Receptors, Cell Surface
Receptors, Thyrotropin
Thyrotropin
Cyclic AMP