Abstract
The effects of acetylcholine (ACh) on the release of [3H]5-hydroxytryptamine ([3H]5-HT) were investigated in synaptosomes prepared from rat cerebral cortex, hypothalamus and hippocampus and depolarized with 15 mM KCl under superfusion conditions. ACh inhibited the release of [3H]5-HT in all three brain areas. This effect was not modified by hexamethonium but was antagonized by atropine and by the non-classical antagonists pirenzepine and dicyclomine.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylcholine / pharmacology
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Animals
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Brain / drug effects
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Brain / physiology
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Cyclohexanecarboxylic Acids / pharmacology*
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Dicyclomine / pharmacology*
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In Vitro Techniques
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Pirenzepine / pharmacology*
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Rats
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Rats, Inbred Strains
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Receptors, Muscarinic / drug effects*
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Receptors, Muscarinic / physiology
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Serotonin / metabolism*
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Synaptosomes / drug effects
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Synaptosomes / physiology
Substances
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Cyclohexanecarboxylic Acids
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Receptors, Muscarinic
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Serotonin
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Pirenzepine
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Dicyclomine
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Acetylcholine