Abstract
In situ hybridization histochemistry of transverse sections from male rat kidney showed that the mRNA of the regulatory enzyme of polyamine degradation, spermidine/spermine N1-acetyltransferase, has a spotty distribution in the cortex, is low and diffused in the outer stripe and high and diffused in the inner stripe of the outer medulla. At the cellular level, this mRNA is solely expressed by the epithelium of the distal straight and convoluted nephron tubules. Since biosynthetic ornithine decarboxylase mRNA is solely found in the proximal straight tubules, it is proposed that polyamine biosynthesis and degradation occur at separate sites along the nephron.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyltransferases / genetics
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Acetyltransferases / isolation & purification*
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Animals
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Base Sequence
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In Situ Hybridization
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Kidney / anatomy & histology
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Kidney / enzymology*
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Kidney Cortex / enzymology
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Kidney Medulla / enzymology
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Male
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Molecular Sequence Data
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Oligonucleotide Probes
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Ornithine Decarboxylase / genetics
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Ornithine Decarboxylase / isolation & purification*
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Polyamines / metabolism
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RNA, Messenger / isolation & purification*
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Rats
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Rats, Wistar
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Tissue Distribution
Substances
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Oligonucleotide Probes
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Polyamines
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RNA, Messenger
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Acetyltransferases
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diamine N-acetyltransferase
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Ornithine Decarboxylase