Abstract
Calcitonin gene-related peptide (CGRP) is a 37 residue neuropeptide that is distantly related to adrenomedullin. We have recently reported the cloning and expression of an adrenomedullin receptor which is approximately 30% homologous to the canine orphan receptor RDC-1. Therefore we tested the hypothesis that RDC-1 was a CGRP receptor. The RDC-1 gene was expressed in COS-7 cells and showed a dose dependant increase of cAMP in response to CGRP and adrenomedullin (EC50 values of 3 x 10(-9) M and 1 x 10(-7) M respectively) which was inhibited by the CGRP antagonist CGRP[8-37]. There was no cAMP response to amylin or [Cys(acm)2,7]a-hCGRP. Ligand binding studies confirmed high affinity of this receptor for CGRP and CGRP[8-37] with KD values of 9.2 x 10(-9) M and 13.4 x 10(-9) M respectively.
MeSH terms
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Adrenomedullin
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Animals
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Base Sequence
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Calcitonin Gene-Related Peptide / metabolism*
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Cells, Cultured
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Chlorocebus aethiops
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Cyclic AMP / metabolism
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DNA Primers / chemistry
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DNA, Complementary / genetics
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Dogs
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Gene Expression
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Peptides / metabolism
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RNA, Messenger / genetics
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Rats
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Receptors, Adrenomedullin
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Receptors, Calcitonin Gene-Related Peptide / genetics*
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Receptors, Calcitonin Gene-Related Peptide / metabolism
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Receptors, Cell Surface / metabolism*
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Receptors, Peptide*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Signal Transduction
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Transfection
Substances
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DNA Primers
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DNA, Complementary
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Membrane Proteins
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Peptides
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RNA, Messenger
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Receptors, Adrenomedullin
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Receptors, Calcitonin Gene-Related Peptide
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Receptors, Cell Surface
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Receptors, Peptide
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Adrenomedullin
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Cyclic AMP
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Calcitonin Gene-Related Peptide