Abstract
EAAC1 is a neuronal and epithelial high affinity glutamate transporter previously cloned from rabbit intestine. Here we report the isolation of EAAC 1 from rat brain* and its expression in the central nervous system based on in situ hybridization. Strong signals were detected in brain, spinal cord and retina. Expression of EAAC1 was particularly strong in pyramidal cells of the cerebral cortex, pyramidal cells of the hippocampus, mitral cells of the olfactory bulb, various thalamic nuclei and cells of certain retinal layers. EAAC1 was also expressed in non-glutamatergic neurons such as GABAergic cerebellar Purkinje cells and alpha-motor neurons of the spinal cord. We propose that EAAC1 is not only involved in the sequestration of glutamate at glutamatergic synapses and in protecting neurons from glutamate excitotoxicity, but also in the cellular metabolism involving glutamate.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Amino Acid Transport System X-AG*
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Animals
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Biological Transport
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Blotting, Northern
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Brain Chemistry
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Carrier Proteins / genetics
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Carrier Proteins / isolation & purification
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Carrier Proteins / metabolism*
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Central Nervous System / cytology
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Central Nervous System / metabolism*
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Cerebral Cortex / drug effects
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Cerebral Cortex / metabolism
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DNA, Complementary / biosynthesis
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DNA, Complementary / isolation & purification
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Excitatory Amino Acid Transporter 3
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Glutamate Plasma Membrane Transport Proteins
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Glutamic Acid / metabolism*
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In Situ Hybridization
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Male
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Molecular Sequence Data
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Neurons / metabolism*
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Polymerase Chain Reaction
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RNA, Messenger / biosynthesis
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Rats
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Rats, Sprague-Dawley
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Retina / cytology
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Retina / metabolism
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Sodium / metabolism
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Spinal Cord / cytology
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Spinal Cord / metabolism
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Symporters*
Substances
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Amino Acid Transport System X-AG
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Carrier Proteins
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DNA, Complementary
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Excitatory Amino Acid Transporter 3
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Glutamate Plasma Membrane Transport Proteins
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RNA, Messenger
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Slc1a1 protein, rat
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Symporters
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Glutamic Acid
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Sodium