Abstract
A novel human GPCR gene was cloned by PCR with degenerate primers designed from cannabinoid type 1 receptor (CB1) sequences and a full-length clone was isolated by screening of a human genomic DNA library. This gene, termed EDG-3, is highly homologous (51.9 % overall and 69.2 % in seven transmembrane regions) to human EDG-1, 47.9 % to rat AGR16 but only 28.0 % to human CB1 receptor. The Northern hybridization analysis showed that a 2.8 kb transcript of EDG-3 is abundantly expressed in the heart followed by placenta, kidney and liver. The EDG-3 gene was mapped on the human chromosome 9q22.1-q22.2 by fluorescence in situ hybridization analysis. Although the ligand and physiological role of this receptor is unknown, this gene may be a new member of the EDG family.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Chromosome Mapping
-
Chromosomes, Human, Pair 9*
-
Cloning, Molecular
-
DNA Primers
-
DNA-Binding Proteins / biosynthesis*
-
DNA-Binding Proteins / chemistry
-
DNA-Binding Proteins / genetics*
-
Female
-
Genomic Library
-
Humans
-
I-kappa B Proteins*
-
In Situ Hybridization, Fluorescence
-
Kidney / metabolism
-
Liver / metabolism
-
Molecular Sequence Data
-
Myocardium / metabolism
-
NF-KappaB Inhibitor alpha
-
Organ Specificity
-
Placenta / metabolism
-
Polymerase Chain Reaction
-
Pregnancy
-
Rats
-
Receptors, Cannabinoid
-
Receptors, Drug / genetics
-
Receptors, Lysophospholipid
-
Recombinant Proteins / biosynthesis
-
Sequence Homology, Amino Acid
-
Sheep
-
Transcription, Genetic
Substances
-
DNA Primers
-
DNA-Binding Proteins
-
I-kappa B Proteins
-
NFKBIA protein, human
-
Nfkbia protein, rat
-
Receptors, Cannabinoid
-
Receptors, Drug
-
Receptors, Lysophospholipid
-
Recombinant Proteins
-
NF-KappaB Inhibitor alpha