Abstract
Brown adipose tissue (BAT) and skeletal muscle are important sites of nonshivering thermogenesis. The uncoupling protein-1 (UCP1) is the main effector of nonshivering thermogenesis in BAT and the recently described ubiquitous UCP2 [1] has been implicated in energy balance. In an attempt to better understand the biochemical events underlying nonshivering thermogenesis in muscle, we screened a human skeletal muscle cDNA library and isolated three clones: UCP2, UCP3L and UCP3S. The novel UCP3 was 57% and 73% identical to human UCP1 and UCP2, respectively, highly skeletal muscle-specific and its expression was unaffected by cold acclimation. This new member of the UCP family is a candidate protein for the modulation of the respiratory control in skeletal muscle.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipose Tissue / metabolism
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Adipose Tissue, Brown / metabolism
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Amino Acid Sequence
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Blotting, Northern
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Body Temperature Regulation / physiology
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Cloning, Molecular
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DNA Probes
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Energy Metabolism
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Gene Expression Regulation*
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Humans
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Ion Channels
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Membrane Proteins / chemistry
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Membrane Proteins / genetics
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Membrane Transport Proteins*
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Mitochondria, Muscle / metabolism*
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Mitochondrial Proteins*
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Molecular Sequence Data
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Muscle, Skeletal / metabolism*
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Proteins / chemistry
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Proteins / genetics
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
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Uncoupling Protein 1
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Uncoupling Protein 2
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Uncoupling Protein 3
Substances
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Carrier Proteins
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DNA Probes
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Ion Channels
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Membrane Proteins
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Membrane Transport Proteins
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Mitochondrial Proteins
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Proteins
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RNA, Messenger
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UCP1 protein, human
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UCP2 protein, human
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UCP3 protein, human
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Uncoupling Protein 1
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Uncoupling Protein 2
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Uncoupling Protein 3
Associated data
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GENBANK/AF035943
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GENBANK/U82818
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GENBANK/U82819
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GENBANK/U84763