Abstract
We have quantified and characterized alpha 1-, alpha 2- and beta-adrenoceptor subtypes in porcine bladder detrusor and bladder neck, human bladder detrusor, and porcine and human prostate. alpha 1-, alpha 2- and beta-adrenoceptor were identified in radioligand binding studies using [3H]prazosin, [3H]RX 821002 and [125I]iodocyanopindolol, respectively, as the radioligands. In porcine male and female detrusor and bladder neck and male prostate, adrenoceptors were detected in the order of abundance beta > alpha 2 >> alpha 1 (not detectable), with no major difference between the sexes or between detrusor and bladder neck. In human detrusor and prostate the order of abundance was beta > alpha 2 >> alpha 1 (not detectable) and beta >> alpha 1 > alpha 2, respectively. The alpha 2-adrenoceptors in all tissues were homogeneously of the alpha 2A-subtype as evidenced by competition binding studies with yohimbine, prazosin, ARC 239 and oxymetazoline. The beta-adrenoceptors represented a mixed population with a dominance of the beta 2-subtype in all tissues as demonstrated by competition binding with ICI 118,551 and CGP 20,712A. We conclude that pigs may be a suitable model for studies of detrusor function with respect to adrenoceptor expression. They may be less suitable for studies of bladder neck or prostate function.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenergic alpha-1 Receptor Agonists
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Adrenergic alpha-1 Receptor Antagonists
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Adrenergic alpha-2 Receptor Agonists
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Adrenergic alpha-2 Receptor Antagonists
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Adrenergic alpha-Agonists / pharmacology
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Adrenergic alpha-Antagonists / pharmacology*
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Adrenergic beta-Antagonists / pharmacology
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Animals
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Binding, Competitive / physiology
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Female
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Humans
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Imidazoles / pharmacology
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Isoquinolines / pharmacology
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Male
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Oxymetazoline / pharmacology
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Piperazines / pharmacology
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Prazosin / pharmacology
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Propanolamines / pharmacology
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Prostate / chemistry*
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Radioligand Assay
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Receptors, Adrenergic / analysis*
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Receptors, Adrenergic, alpha-1 / analysis
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Receptors, Adrenergic, alpha-2 / analysis
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Receptors, Adrenergic, beta / analysis
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Swine
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Urinary Bladder / chemistry*
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Yohimbine / pharmacology
Substances
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Adrenergic alpha-1 Receptor Agonists
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Adrenergic alpha-1 Receptor Antagonists
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Adrenergic alpha-2 Receptor Agonists
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Adrenergic alpha-2 Receptor Antagonists
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Adrenergic alpha-Agonists
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Adrenergic alpha-Antagonists
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Adrenergic beta-Antagonists
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Imidazoles
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Isoquinolines
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Piperazines
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Propanolamines
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Receptors, Adrenergic
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Receptors, Adrenergic, alpha-1
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Receptors, Adrenergic, alpha-2
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Receptors, Adrenergic, beta
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Yohimbine
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ICI 118551
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Oxymetazoline
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CGP 20712A
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AR-C239
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Prazosin