Abstract
Using the signal sequence trap method, we have cloned a novel 12-membrane-spanning transporter-like protein, termed renal-specific transporter (RST), from the mouse kidney. RST is a 553-amino-acid protein highly homologous to recently cloned organic cation transporters, e.g. it is 30% identical to rat organic cation transporter I at the amino acid level. Northern blot analysis has revealed that the RST gene is expressed abundantly and specifically in the kidney. In situ hybridization analysis has shown that RST gene expression is restricted to the renal proximal tubule, where various organic cations such as endogenous catecholamines and choline or clinically used cationic drugs are known to be actively excreted.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Carrier Proteins / biosynthesis*
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Carrier Proteins / chemistry
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Carrier Proteins / genetics
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Cloning, Molecular
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DNA, Complementary
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Gene Library
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Kidney / metabolism*
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Male
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Membrane Proteins / biosynthesis*
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Membrane Proteins / chemistry
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Membrane Proteins / genetics
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Organic Anion Transporters
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Organic Cation Transport Proteins*
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Organic Cation Transporter 2
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Protein Sorting Signals / biosynthesis
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Rats
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Sequence Alignment
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Sequence Homology, Amino Acid
Substances
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Carrier Proteins
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DNA, Complementary
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Membrane Proteins
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Organic Anion Transporters
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Organic Cation Transport Proteins
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Organic Cation Transporter 2
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Protein Sorting Signals
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Slc22a12 protein, mouse
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Slc22a2 protein, mouse
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Slc22a2 protein, rat