Abstract
A novel member of the ionotropic ATP receptor gene family has been identified in human brain. This 422 amino acid long P2X receptor subunit has 62% sequence identity with rat P2X5. Several characteristic motifs of ATP-gated channels are present in its primary structure, but this P2X5-related subunit displays a single transmembrane domain. Heterologous expression of chimeric subunits containing the C-terminal domain of rat P2X5 leads to the formation of desensitizing functional ATP-gated channels in Xenopus oocytes. The developmentally regulated mRNA, found in two splicing variant forms, is expressed at high levels in brain and immune system.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain / metabolism*
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Cerebellum / metabolism
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Female
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Fetus
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Genetic Variation
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Humans
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Lymphocytes / metabolism*
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Macromolecular Substances
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Molecular Sequence Data
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Multigene Family
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Oocytes / physiology
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Organ Specificity
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RNA, Messenger / biosynthesis
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Rats
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Receptors, Purinergic P2 / biosynthesis*
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Receptors, Purinergic P2 / chemistry*
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Sequence Alignment
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Sequence Deletion
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Sequence Homology, Amino Acid
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Transcription, Genetic
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Xenopus laevis
Substances
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Macromolecular Substances
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RNA, Messenger
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Receptors, Purinergic P2