It's not just a phase: crystallization and X-ray structure determination of bacteriorhodopsin in lipidic cubic phases

E Gouaux

doi:10.1016/s0969-2126(98)00002-1

It's not just a phase: crystallization and X-ray structure determination of bacteriorhodopsin in lipidic cubic phases

Structure. 1998 Jan 15;6(1):5-10. doi: 10.1016/s0969-2126(98)00002-1.

Author

E Gouaux¹

Affiliation

¹ Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA. jeg52@columbia.edu

PMID: 9493262
DOI: 10.1016/s0969-2126(98)00002-1

Abstract

Utilization of lipidic cubic phases in the crystallization of bacteriorhodopsin (bR) has yielded long sought after crystals that diffract X-rays to 2 A resolution. The resulting structure provides new information on the protein conformation and the mechanism of proton translocation. Crystallization of bR via lipidic cubic phases may be a harbinger of new membrane protein crystallization strategies.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
Review

MeSH terms

Bacterial Proteins / chemistry
Bacteriorhodopsins / chemistry*
Crystallization
Crystallography, X-Ray
Halobacterium salinarum / chemistry*
Membrane Proteins / chemistry
Models, Molecular

Substances

Bacterial Proteins
Membrane Proteins
Bacteriorhodopsins