Abstract
ATP and UTP did not evoke [Ca2+]i signals in rat foetal lung epithelial cells grown on glass but elicited clear responses in cells grown into functionally polarised epithelia on permeable supports. Moreover, P2Y2 receptor mRNA could not be detected in cells on glass by the polymerase chain reaction but this mRNA species was clearly expressed by polarised cells. P2Y2 receptor expression thus appears to be a feature of the polarised phenotype.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / pharmacology
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Animals
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Calcium / physiology
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Cell Polarity
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Cells, Cultured
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Epithelial Cells / drug effects
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Epithelial Cells / metabolism*
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Epithelial Cells / physiology
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Fetus / metabolism
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Glass
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Lung / cytology
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Lung / drug effects
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Lung / embryology
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Lung / metabolism*
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Rats
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Receptors, Purinergic P2 / biosynthesis*
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Receptors, Purinergic P2Y2
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Uridine Triphosphate / pharmacology
Substances
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P2ry2 protein, rat
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Receptors, Purinergic P2
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Receptors, Purinergic P2Y2
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Adenosine Triphosphate
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Calcium
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Uridine Triphosphate