Electron microscopy remains one of the most accurate methods for the detection of autophagy
and quantification of autophagic accumulation. Compared to fluorescence microscopy, …

Autophagosomes have been reported to form in the vicinity of the endoplasmic reticulum (ER).
In many cases, the phagophore membrane is observed between two cisternae of rough …

Transplantation of pancreatic islet cells derived from human pluripotent stem cells is a promising
treatment for diabetes. Despite progress in the generation of stem-cell-derived islets (SC…

Modern research in the life sciences is unthinkable without computational methods for
extracting, quantifying and visualising information derived from microscopy imaging data of …

The actin cytoskeleton plays a fundamental role in various motile and morphogenetic
processes involving membrane dynamics. We show that actin-binding proteins MIM (missing-in-…

Plant cells are connected through plasmodesmata (PD), membrane-lined channels that allow
symplastic movement of molecules between cells. However, little is known about the role …

The organization of multiple mitochondrial DNA (mtDNA) molecules in discrete protein-DNA
complexes called nucleoids is well studied inSaccharomyces cerevisiae. Similar structures …

Tissue homeostasis requires maintenance of functional integrity under stress. A central
source of stress is mechanical force that acts on cells, their nuclei, and chromatin, but how the …

Understanding the structure–function relationship of cells and organelles in their natural
context requires multidimensional imaging. As techniques for multimodal 3-D imaging have …

Seipin is an endoplasmic reticulum ( ER ) membrane protein implicated in lipid droplet ( LD
) biogenesis and mutated in severe congenital lipodystrophy ( BSCL 2). Here, we show that …